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通过NAC转录因子对PECTATE LYASE5的升级促进了果中的水果软化
Qiufang Su1, Huijuan Yang1, Xianglu Li1
1State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Shaanxi 712100, China.
在PECTATE LYASE5 (MdPL5) 基因附近的转子体插入抑制了其表达,导致果更加坚固. 这一发现有助于开发果育种计划的分子标记物.
科学领域:
- 植物遗传学 植物遗传学
- 果实质量研究 果实质量研究
- 分子育种是一种分子育种.
背景情况:
- 果肉的坚固性对于果的消费者吸引力,保质期和可运输性至关重要.
- 果肉坚硬性的遗传基础尚未完全理解.
- 了解坚固性遗传学是标记器辅助育种的关键.
研究的目的:
- 为了研究果肉坚的遗传基础.
- 为了识别分子标记物,用于培育更坚固的果品种.
- 为了阐明监管网络控制水果坚硬性.
主要方法:
- 对439种F1果杂交品种进行表型分析,以测量肉的坚硬度.
- 大量分离分析 (BSA) 与RNA测序相结合.
- 下一代重新测序以识别遗传变异.
- 基因表达分析和促进体研究.
主要成果:
- 在PECTATE LYASE5 (MdPL5) 上游的Harbinger-like转子体插入与增加的肉度有关.
- 这种转位子插入抑制了MdPL5的表达,与一个非常硬的表型相关.
- MdNAC1-L被确定为一种转录激活剂,可对MdPL5和其他与软化相关的基因进行上调.
结论:
- 与可移植元素 (TE) 相关的遗传变异显著影响果肉坚硬度.
- MdPL5在水果软化中发挥作用,其表达受到MdNAC1-L.L的调节.
- 这些发现提供了对控制果坚硬性的分子机制的见解,并为繁殖提供了工具.
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