在cis-regulatory元素之间的改变的相互作用部分解决了Capsella rubella中的BLADE-ON-PETIOLE遗传冗余性
Thi Chi Tran1, Karoline Mähl1, Christian Kappel1
1Institute for Biochemistry and Biology, University of Potsdam, Potsdam-Golm D-14476, Germany.
The Plant cell
|August 19, 2024
概括
基因重复允许进化的创新. 在红色牧羊人的钱包中,BLADE-ON-PETIOLE 1 (BOP1) 基因促进物的改变相互作用导致了基因冗余的丧失和改变了花朵的发育.
科学领域:
- 植物进化遗传学 植物进化遗传学
- 分子进化是分子进化的过程.
- 发育生物学是发展生物学.
背景情况:
- 基因重复是进化新性的主要来源,导致新功能化,次功能化或伪基因化.
- 复制后基因表达模式的差异通常被解释为子功能化,但它们的功能和分子基础仍然不清楚.
- 叶片上皮层 (BOP) 基因BOP1和BOP2为研究冗余性损失提供了一个模型.
研究的目的:
- 与Arabidopsis thaliana相比,调查Capsella rubella中BOP1和BOP2之间的部分冗余损失的遗传和分子基础.
- 了解C. rubella.中改变BOP1表达的功能影响.
- 识别控制BOP1表达的Cis调节元素及其相互作用.
主要方法:
- 在Capsella rubella和Arabidopsis thaliana中对BOP1和BOP2进行比较分析.
- 在Arabidopsis thaliana中进行的转基因救援实验atbop1atbop2双重突变.
- 在内源的AtBOP1促进体中删除,以评估cis-regulatory元素的功能.
- 在BOP1促进体内对保存的非编码序列的分析.
主要成果:
- 在C. rubella中,BOP1在隐秘的胸膜原始体中表现出改变的表达模式,导致花器官发育受损,并无法抑制胸膜形成.
- 多个BOP1促进子区域,包括保存的非编码序列,以非添加的方式相互作用,以调节原体中的表达.
- 这些Cis调节相互作用的变化是C. rubella和A. thaliana之间的BOP1表达和活性进化差异的基础.
- 改变的调节相互作用也解释了BOP1在花器官脱离中的作用的分歧.
结论:
- 植物中的促进器架构复杂,涉及ciss-regulatory元素之间的复杂相互作用.
- 调节基因元素相互作用的变化是基因表达进化差异的关键驱动因素.
- 这些变化在进化过程中对基因冗余的丧失有显著的贡献.
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