使用多孔石墨碳柱和液态染色体质谱法分析人类生物样本:一篇综述
Taís Betoni Rodrigues1, Ricardo Leal Cunha2, Paulo Emílio Pereira Barci3
1Laboratory of Chromatography (CROMA), São Carlos Institute of Chemistry, University of São Paulo (USP), São Carlos, São Paulo, 13560-970, Brazil. tais.betoni.rodrigues@usp.br.
多孔石墨碳 (PGC) 柱为液态染色学-质谱学 (LC-MS) 分析人类生物样本提供了独特的优势. 这次审查突出了PGC的重点.
科学领域:
- 分析化学 分析化学
- 生物医学分析 医学分析
- 染色体学 染色体学 是一种染色学.
背景情况:
- 液体染色学-质谱学 (LC-MS) 对于分析复杂的生物样本至关重要.
- 多孔石墨碳 (PGC) 柱为极性和非极性化合物提供独特的分离能力.
- PGC柱在广泛的pH范围和高温下表现出极好的稳定性,提高了LC-MS兼容性.
研究的目的:
- 审查PGC用于固体相提取 (SPE) 和分离在人类生物样本LC-MS分析中的适用性.
- 突出PGC-LC-MS在分析代谢物,药品和生物标志物的各种应用.
- 探索LC-MS中PGC列的原则,优势,挑战和方法开发.
主要方法:
- 对LC-MS中PGC列应用的现有文献的审查.
- 关于PGC柱化学和属性的讨论.
- 在人类生物样本分析中对PGC-LC-MS应用的分析.
主要成果:
- PGC柱有效地分离各种分析物,包括甘氨酸,蛋白质,寡糖,糖酸盐和核酸.
- PGC-LC-MS证明了对内源代谢产物,药品和生物标志物的广泛适用性.
- 对于LC-MS来说,PGC柱在稳定性和广泛的分析器兼容性方面具有优势.
结论:
- PGC列是基于LC-MS对人类生物样本的分析的一个有价值的工具.
- 了解PGC的能力和局限性有助于分析方法的进步.
- 本综述是生物医学研究的研究人员和从业人员的资源.
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