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相关概念视频

CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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相关实验视频

Updated: Jun 16, 2025

Embryo Microinjection and Knockout Mutant Identification of CRISPR/Cas9 Genome-Edited Helicoverpa Armigera Hübner
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使用反CRISPR-Cas12a系统的多功能植物基因组工程.

Yao He1,2, Shishi Liu2,3, Long Chen4,5

  • 1Integrative Science Center of Germplasm Creation in Western China (Chongqing) Science City, Chongqing Key Laboratory of Tree Germplasm Innovation and Utilization, School of Life Sciences, Southwest University, Chongqing, 400715, China.

Science China. Life sciences
|August 19, 2024
PubMed
概括

科学家们为植物建立了一个抗CRISPR-Cas12a系统,使用AcrVA1控制基因组编辑. 该系统减少了非目标突变,微调了编辑效率,并实现了可诱导,组织特定的编辑和合成基因电路.

关键词:
在 AcrVA1 中,AcrVA1 是克里斯普拉是一个.这就是Cas12a.这是一个反-CRISPR.微调的基因组编辑.可诱导和组织特定的基因组编辑.非目标效应的非目标效应.合成逻辑电路 合成逻辑电路

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相关实验视频

Last Updated: Jun 16, 2025

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High-throughput CRISPR Vector Construction and Characterization of DNA Modifications by Generation of Tomato Hairy Roots

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科学领域:

  • 植物生物技术 植物生物技术
  • 基因组工程是基因组工程.
  • 分子生物学分子生物学

背景情况:

  • 克里斯普尔-卡斯12a系统是植物研究和作物改良的重要工具.
  • 反CRISPR-Cas12a系统在植物中的应用和有效性仍未得到充分研究.

研究的目的:

  • 为了识别和描述抑制植物Cas12a活性的抗CRISPR蛋白质.
  • 开发一种多功能抗CRISPR系统,用于植物精确的基因组工程.

主要方法:

  • 在分析中识别潜在的抗CRISPR蛋白质.
  • 在体外和植物内测试以评估抑制Cas12a活性.
  • 全基因组测序以评估非目标效应.
  • 转基因植物的生成用于诱导性和组织特异性基因编辑.

主要成果:

  • 在体外,AcrVA1显示出强大的Mb2Cas12a和LbCas12a抑制.
  • 在大米原生体和转基因系中,AcrVA1有效抑制了LbCas12a介导的基因组编辑.
  • 同时表达AcrVA1显著减少了由CRISPR-LbCas12a诱导的目标外突变.
  • 控制AcrVA1表达使可诱导的,组织特异的基因组编辑和合成逻辑门用于基因调节.

结论:

  • 已经建立了一个有效的抗CRISPR-Cas12a系统用于植物应用.
  • 该系统提供对基因组编辑的精确控制,减轻非目标效应,并使编辑效率的微调成为可能.
  • 开发的系统有助于对基因组编辑和在植物中创建合成基因电路的时空控制.