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相关概念视频

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.

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相关实验视频

Updated: Jul 19, 2026

Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera
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集成主动灭电路用于基于SPAD的时间分辨率光应用的高速率和无扭曲.

Francesco Malanga, Gennaro Fratta, Giulia Acconcia

    IEEE transactions on biomedical circuits and systems
    |August 19, 2024
    PubMed
    概括

    本研究引入了一个主动灭电路 (AQC),以克服时间相关单光子计数 (TCSPC) 中的堆积效应. 这种新型电路使得即使在高光子速率下也可以进行无扭曲测量,从而提高了FLIM等应用中的灵敏度.

    科学领域:

    • 仪器仪表与测量科学 仪器仪表与测量科学
    • 光子学和光学工程的工程.

    背景情况:

    • 时间相关单光子计数 (TCSPC) 对于光终生成像显微镜 (FLIM) 和量子光学中低光检测至关重要.
    • 堆积效应在高光子检测速率下显著扭曲TCSPC测量,从而限制了性能.
    • 现有的堆积解决方案往往涉及复杂的后处理或多通道系统,使设置复杂化.

    研究的目的:

    • 开发一种新的主动灭电路 (AQC),以减轻TCSPC中的堆积效应.
    • 通过精确控制光探测器死亡时间,在高照明条件下实现无扭曲的TCSPC直方图.
    • 为了确保强大而精确的定时调节,耐环境变化.

    主要方法:

    • 使用高压150nm技术开发一个主动火电路 (AQC).
    • 实现过程,电压和温度 (PVT) 补偿,以实现稳定的死亡时间控制.
    • 在高计数率条件下使用光测量进行实验验证.

    主要成果:

    • 该AQC实现了50 ps的死时分辨率,适用于从20到100 MHz的激光频率.
    • 在不同的条件下,在截止时间控制中表现出接近理想的线性.
    • 实验结果显示,在高计数率条件下,扭曲率低至0.43%.

    更多相关视频

    Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals
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    Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
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    Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

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    相关实验视频

    Last Updated: Jul 19, 2026

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    06:08

    Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera

    Published on: December 27, 2018

    8.8K
    Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals
    00:07

    Excitation-Scanning Hyperspectral Imaging Microscopy to Efficiently Discriminate Fluorescence Signals

    Published on: August 22, 2019

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    Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
    14:12

    Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

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    结论:

    • 开发的AQC有效地克服了TCSPC中的堆积限制.
    • 这一进步使要求更高的光学应用中能够进行更准确,更灵敏的测量.
    • 该电路为时间解决的实验提供了强大而精确的解决方案.