Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关实验视频

Updated: Jun 16, 2025

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
10:50

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

Published on: April 1, 2016

10.9K

使用合成酵母选择系统演化双特征EPSP合成酶变体.

Kevin B Reed1, Wantae Kim1, Hongyuan Lu1

  • 1McKetta Department of Chemical Engineering, The University of Texas at Austin, Austin, TX 78712.

Proceedings of the National Academy of Sciences of the United States of America
|August 19, 2024
PubMed
概括

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Recent advances in antiviral drugs for Chikungunya virus (CHIKV): Targets, mechanisms, and development strategies.

Acta pharmaceutica Sinica. B·2026
Same author

Engineering Biosensors to Enhance Monoterpene Indole Alkaloid Production in Yeast.

bioRxiv : the preprint server for biology·2026
Same author

A tunable CTD grammar governs the spatial programming of the transcription cycle.

Nature communications·2026
Same author

A Linear Mixed Effects Model for Evaluating Synthetic Gene Circuits.

ACS synthetic biology·2026
Same author

Comparison of the clinical efficacy, safety and EEG functional connectivity changes between 18-Hz rTMS and iTBS of accelerated dTMS treatment for major depressive disorder: a randomized controlled trial.

Molecular psychiatry·2026
Same author

DKK1 Suppresses Hippo Signaling via PIP<sub>3</sub>-OGT-LRP6 <i>O</i>-GlcNAcylation in Hepatocellular Carcinoma.

Cancer communications (London, England)·2026

研究人员开发了一种合成酵母系统,以进化5-enolpyruvylshikimate-3-phosphate合成酶 (EPSPS) 以提高草甘耐受性和催化效率. 一个单一的进化周期产生了具有强大的除草剂耐药性和改善酶功能的突变物.

科学领域:

  • 生物化学 生物化学
  • 植物科学 植物科学
  • 合成生物学 合成生物学

背景情况:

  • 5enolpyruvylshikimate-3-phosphate合成酶 (EPSPS) 对于植物中的芳香氨基酸合成至关重要,并且是草甘除草剂的目标.
  • 现有的抗草甘的EPSPS变体往往表现出减少的催化效率,需要新的变体与双特征改进.

研究的目的:

  • 建立一个合成酵母系统,以快速进化EPSPS,具有高的草甘耐受性和催化效率.
  • 为了确定克服除草剂耐药性和酶性能之间的典型权衡的新型EPSPS突变.

主要方法:

  • 创建了一个合成酵母系统,用于研究和演化异质EPSP合成酶.
  • 该系统使用已知的EPSPS变体进行了验证,以确认草甘应激下植物生长特征的总结.
  • 突变性图书馆在强烈的双特征选择压力下进行了选.

主要成果:

  • 合成酵母系统成功地回顾了植物对草甘的生长反应.
  • 在双特征选择压力下的单一进化周期确定了一个优越的EPSPS突变.
  • 与起始酶相比,进化突变体表现出强大的草甘耐受性 (Ki ~1 mM) 和酶效率增加了约2.5倍.
  • 水晶结构揭示了对突变的洞察力,赋予了改进的双重特征.
关键词:
在 EPSPSPS中使用.指导进化是指导进化的.草甘是一种草甘.合成生物学 合成生物学酵母酵母是一种酵母.

更多相关视频

Protein Engineering by Yeast Surface Display
05:49

Protein Engineering by Yeast Surface Display

Published on: November 29, 2024

1.2K
Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli
09:01

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli

Published on: March 16, 2011

30.5K

相关实验视频

Last Updated: Jun 16, 2025

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening
10:50

Directed Evolution Method in Saccharomyces cerevisiae: Mutant Library Creation and Screening

Published on: April 1, 2016

10.9K
Protein Engineering by Yeast Surface Display
05:49

Protein Engineering by Yeast Surface Display

Published on: November 29, 2024

1.2K
Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli
09:01

Mutagenesis and Functional Selection Protocols for Directed Evolution of Proteins in E. coli

Published on: March 16, 2011

30.5K

结论:

  • 开发的合成酵母平台对进化EPSPS有效,具有改善的草甘耐药性和催化效率.
  • 确定了新的EPSPS突变,它们有可能在全植物应用中优于现有的耐药变体.
  • 这个平台有助于探索EPSPS中草甘耐药性和酶效率之间的复杂关系.