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皇冠以太剂可减少离子抑制并改善液体微聚合物表面采样探头的检测
Haidy Metwally1, Jian Yu1, Rachel Theriault2
1Department of Chemistry, Queen's University, Kingston, Ontario, Canada.
Rapid communications in mass spectrometry : RCM
|August 19, 2024
概括
皇冠 (CEs) 降低了和污染物在亚甘上培养的海洋细菌的质谱分析. 这种方法提高了光谱质量,并促进了快速的微生物代谢物分析,以发现天然产品.
科学领域:
- 分析化学 分析化学
- 微生物学 微生物学
- 自然产品的发现自然产品的发现
背景情况:
- 和对于agar介质上的海洋细菌生长至关重要,但在质谱学中会造成严重的污染.
- 酸盐会导致离子抑制和 adduct 形成,降低光谱质量并阻碍代谢物分析.
- 直接采样细菌殖民地在阿加减少样本的准备,但可以加剧污染问题.
研究的目的:
- 开发一种方法来隔离和离子在细菌殖民地直接质谱采样期间.
- 为了减少背景干扰并改善微生物代谢物分析的光谱质量.
- 为了更快,更准确地检测天然产品发现的化合物.
主要方法:
- 使用液体微聚合表面采样探头 (LMJ-SSP) 的冠 (CE) 直接采样细菌殖民地.
- 在LMJ-SSP的载体溶剂 (甲醇或甲醇:H2O) 中应用CE (18-皇冠-6或15-皇冠-5).
- 在CE添加之前和之后使用信息理论措施研究了光谱变化.
主要成果:
- 证明了CE在减少细菌殖民地直接分析期间背景干扰的有效性.
- 在Pseudoalteromonas rubra和Pseudoalteromonas tunicata中观察到盐背景的缓解和改进的化合物检测.
- 通过CE应用展示了减少的化学噪声和增强的光谱可解释性.
结论:
- 开发的方法有效地减少了细菌殖民地的质谱学中的酸盐干扰.
- 与LMJ-SSP相结合的皇冠乙烯为更清洁的直接采样和分析提供了可行的解决方案.
- 这种方法支持通过快速准确的化合物检测,有效地发现天然产品.
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