探索挥发性化合物和微生物动力学:Kluyveromyces marxianus和Hanseniaspora opuntiae可以减少Forastero可可发酵时间
Lizbeth M Coria-Hinojosa1, Dulce Velásquez-Reyes1, Montserrat Alcázar-Valle1
1Food Technology Department, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco (CIATEJ), A.C., Camino Arenero 1227, 45019 El Bajío, Zapopan, Jalisco, Mexico.
Food research international (Ottawa, Ont.)
|August 19, 2024
概括
使用Kluyveromyces marxianus等非可可酵母作为起始培养可以提高可可发酵的一致性. 这种方法增强了芳香的产生,并减少了乙酸,以获得更好的风味.
科学领域:
- 食品科学 食品科学 食品科学
- 微生物学 微生物学
- 发酵技术的发酵技术
背景情况:
- 传统的可可发酵是自发的,导致可变的感官质量.
- 酵母初始培养为可可豆提供了一条通往一致的感官特征的途径.
研究的目的:
- 评估来自非可可来源的Hanseniaspora opuntiae和Kluyveromyces marxianus作为可可发酵的起始作物.
- 在可可发酵过程中将微生物种群与挥发性化合物概况相关联.
主要方法:
- 高通量DNA测序以识别主导酵母.
- 气色谱-质谱学 (GC-MS) 与固相微提取 (HS-SPME) 结合,用于分析挥发性化合物.
- 多变量统计分析 (主要组件分析,聚合层次聚类) 用于相关性.
主要成果:
- 克劳维罗米塞斯马克西安纳斯被确定为主要的酵母.
- 确定了63种挥发性化合物,并与微生物丰富度相关联.
- 这些酵母的使用增加了芳香质的形成,并降低了酸含量.
结论:
- 来自其他发酵过程的酵母是可可可开始培养的可行候选者.
- 这种方法可以带来更好的,更一致的可可豆香味和质量.
相关概念视频
Fermentation
Most eukaryotic organisms require oxygen to survive and function adequately. Such organisms produce large amounts of energy during aerobic respiration by metabolizing glucose and oxygen into carbon dioxide and water. However, most eukaryotes can generate some energy in the absence of oxygen by anaerobic metabolism.
Fermentation is a type of metabolic process that occurs in the absence of oxygen, where organic molecules such as glucose are broken down to produce energy. During this process, the...
Fermentation is a type of metabolic process that occurs in the absence of oxygen, where organic molecules such as glucose are broken down to produce energy. During this process, the...
Microbial Fermentation
Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
Microbes in Food Production
Microbial fermentation is central to food biotechnology, enhancing flavor, texture, preservation, and stability. Fermentative microorganisms metabolize carbohydrates into organic acids, alcohols, and other metabolites that inhibit spoilage organisms and improve digestibility while contributing distinctive sensory qualities.In baking, amylases naturally present in flour hydrolyze starch into monosaccharides such as glucose, which Saccharomyces cerevisiae ferments anaerobically. Through...
Microbes in Beverage Production
Alcoholic beverages such as wine, beer, and spirits are the products of microbial fermentation processes that transform simple sugars into ethanol and a wide array of complex flavor compounds. These transformations rely on the metabolic activities of specific yeasts and bacteria, which are selected and controlled to yield the desired beverage characteristics.Wine Fermentation and MaturationWine production begins with the crushing of grapes to release juice and pulp, forming a must that is...
Microbes in the Production of Fermented Foods
Lactic acid bacteria (LAB) and molds are instrumental in fermenting plant-based foods to enhance preservation and ensure year-round availability. These microbial processes convert plant carbohydrates into organic acids and other metabolites that inhibit spoilage organisms and contribute to the sensory qualities of the final product.In sauerkraut production, cabbage goes through a microbial succession that starts with cocci such as Leuconostoc mesenteroides. These microbes begin fermentation by...
Bioreactor Controls-III
Strain improvement is a foundational strategy in industrial microbiology aimed at maximizing microbial productivity, particularly because natural isolates typically yield commercially valuable products in very low concentrations. Although optimizing the culture medium and environmental conditions can improve yields, these adjustments are inherently limited by the organism’s genetic potential. As a result, the focus shifts toward genetic modifications to enhance biosynthetic capacity. The...


