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评估使用Caco-2和人类iPS细胞衍生的小肠上皮细胞在素水解中对Met-Lys-Pro的肠道吸收

Takumi Yago¹, Naoki Yuda¹, Miyuki Tanaka¹

¹Innovative Research Institute, Morinaga Milk Industry Co., Ltd., 5-1-83, Higashihara, Zama-City, Kanagawa-Pref. 252-8583, Japan.

Food research international (Ottawa, Ont.)

|August 19, 2024

概括

每天从素水解盐中摄取Met-Lys-Pro (MKP) 可以降低血压. 这项研究发现,氨酸化物中的MKP比合成MKP更好地吸收,这表明其生物可用性有所改善.

科学领域:

生物化学生物化学
胃肠病学胃肠病学
心血管研究研究心血管研究

背景情况:

高血压是心血管疾病的重要危险因素.
以前的研究表明,含有Met-Lys-Pro (MKP) 的素解剂可以安全降低轻度高血压.
了解MKP的肠道吸收对于其治疗应用至关重要.

研究的目的:

为了比较Met-Lys-Pro (MKP) 的肠道吸收,从素水解与合成MKP.
研究Caco-2细胞和人类诱导的多能干细胞衍生小肠上皮细胞 (hiSIECs) 在预测吸收中的作用.

主要方法:

使用Caco-2细胞单层和hiSIECs来模拟肠道吸收.
测量了完整的MKP的透系数 (Papp).
在不同的条件下评估完整的MKP的跨甲状腺运输和残留比率.

主要成果:

在Caco-2细胞和hiSIECs中,MKP被完好无损地运输.
对于MKP,透系数 (Papp) 是0.57±0.14×10−7cm/s (Caco-2) 和1.03±0.44×10−7cm/s (hiSIECs) 的.
与合成MKP相比,素水解剂显著增加了完整的MKP运输和残留比率,表明降低了降解和增强了吸收.

结论:

hiSIECs显示出预测生物活性的肠道吸收的潜力.
摄入MKP作为素水解剂的一部分可能会提高其生物可用性,而不是单独合成MKP.
素水解酸中的其他的存在似乎可以保护MKP免受降解,并促进其运输.

关键词:

生物活性的生物活性.生物可用性生物可用性在Caco-2细胞单层中.素水解酸是因为素的水解.人类iPS细胞衍生的小肠上皮细胞细胞.穿越皮质的运输.

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相关概念视频

Protein Absorption

Protein Absorption

Proteins in the gastrointestinal tract typically come from food, but they can also originate from disintegrated cells or secreted enzymes. In the stomach, the enzyme pepsin breaks down these proteins into polypeptides. The fragments then move into the duodenum as a semi-fluid mass called chyme. Pancreatic proteases, such as trypsin and chymotrypsin, and intestinal brush border enzymes like carboxypeptidases further dismantle the polypeptides into tripeptides, dipeptides, and free amino acids.

Protein Digestion

Protein Digestion

Protein digestion begins in the stomach, where the highly acidic environment can easily disrupt protein structure by exposing the peptide bonds of polypeptide chains. After polypeptide chains are broken into individual amino acids by a series of digestive enzymes, the amino acids are transported to the liver via the bloodstream to produce energy.