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Updated: Jun 16, 2025

Label-free Single Molecule Detection Using Microtoroid Optical Resonators
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Label-free Single Molecule Detection Using Microtoroid Optical Resonators

Published on: December 29, 2015

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通过microtoroid光学共振器光热显微镜的单个5纳米量子点检测

Shuang Hao1, Sartanee Suebka1, Judith Su2

  • 1Wyant College of Optical Sciences, University of Arizona, Tucson, AZ, 85721, USA.

Light, science & applications
|August 19, 2024
PubMed
概括

这项研究引入了一种高度敏感的无标签方法,使用微粒体共振器和光热显微镜来检测单个5nm量子点 (QD). 该技术实现了异常的信号噪声比,使得用于先进的纳米材料和生物应用的最小散热的检测成为可能.

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Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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