相关实验视频
Updated: Jun 16, 2025

17:14
In vivo and in vitro Studies of Adaptor-clathrin Interaction
Published on: January 26, 2011
14.5K
对早期分泌途径的CHC22克拉林招募需要与SNX5和p11515进行双位点相互作用
Joshua Greig1,2, George T Bates1,2, Daowen I Yin1,2
1Structural and Molecular Biology, Division of Biosciences, University College London, London, WC1E 6BT, UK.
The EMBO journal
|August 19, 2024
概括
克拉特林重链CHC22使用双结合机制在肌肉细胞中运输葡萄糖转运器GLUT4. 这条途径绕过了高尔基细胞,这对于胰岛素调节的葡萄糖吸收至关重要.
科学领域:
- 细胞生物学 细胞生物学
- 分子生物学分子生物学
- 生物化学 生化学
背景情况:
- 两个克拉特林异型,CHC17和CHC22,经营着不同的细胞内运输通路.
- CHC17参与了一般的内细胞和膜交通.
- CHC22主要表达在骨肌肉中,并促进GLUT4转运体的贩运.
研究的目的:
- 确定区分CHC22贩运与CHC17贩运的分子机制.
- 为了阐明CHC22如何针对GLUT4运输的内等质网膜到戈尔吉中间体 (ERGIC).
主要方法:
- 通过生物化学分析研究了蛋白质与蛋白质相互作用.
- 定义了在CHC22局部化中的特定克拉特林域 (N-终端和C-终端) 的作用.
- 利用遗传干扰来评估已识别的相互作用的功能影响.
主要成果:
- CHC22的C端域与SNX5结合,该SNX5与ERGIC带p115.5相互作用.
- 独立于ESCPE-1复合体,SNX5和SNX6对于CHC22定位至关重要.
- 在CHC22上的N端域补丁也结合p115,调解双重招募机制.
- 这种双边相互作用对CHC22向ERGIC膜至关重要,使GLUT4.4的Golgi绕道成为可能.
结论:
- CHC22使用了一种新的两方互动策略来针对ERGIC目标,涉及其N和C端.
- 这种机制对于直接将GLUT4贩运到一个储存区,绕过戈尔吉河而直接贩运至关重要.
- 已确定的途径是人类葡萄糖代谢的关键调节者,特别是在对胰岛素的反应中.
相关概念视频
Clathrin Coated Vesicles
6.9K
Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
6.9K
Pinching-off of Coated Vesicles
3.1K
Vesicle budding is orchestrated by distinct cytosolic proteins such as adaptor proteins, coat proteins, and GTPases. To initiate vesicle budding, membrane-bending proteins containing crescent-shaped BAR domains bind to the lipid heads in the bilayer and distort the membrane to form a protein-coated vesicle bud. Adaptors proteins such as AP2 for clathrin-coated vesicles can nucleate on the deformed membrane. Finally, coat proteins such as clathrin or COPI and COPII assemble into a coat forming...
3.1K
Overview of Secretory Vesicles
8.5K
Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
8.5K
Coat Assembly and GTPases
3.5K
Vesicles incorporate different coat protein subunits in different cell locations, which changes the properties of the coat, such as the shape and geometry of the transport vesicles. Thus, vesicle coat proteins also play a significant role in cargo selection.
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
Coat assembly depends on the local availability of phosphatidylinositol phosphates or PIPs and GTP-binding proteins. Adaptor proteins, which link the coat proteins to the membrane, bind to these PIPs and play a crucial role in controlling...
3.5K
SNAREs and Membrane Fusion
10.8K
Once a transport vesicle has recognized its target organelle, the vesicular membrane needs to fuse with the target membrane to unload the cargo. Transmembrane proteins called SNAREs present on organelle membranes and their vesicles, mediate vesicle fusion.
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
SNAREs exist in pairs that symmetrically interact and catalyze the fusion of the lipid bilayers in vesicle and target organelle. v-SNARE in the vesicle membrane are single polypeptide chains that bind to a complementary t-SNARE, composed of 2...
10.8K
Vesicular Tubular Clusters
2.5K
After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...
With the help of motor proteins such...
2.5K

