微型建筑球体的混沌辅助生产 (CAPAS)
Carlos Fernando Ceballos-González1,2,3, Edna Johana Bolívar-Monsalve1,2,3, Silvana Velásquez-Marín1
1Centro de Biotecnología-FEMSA, Tecnologico de Monterrey, Monterrey, NL, 64849, México.
Small (Weinheim an der Bergstrasse, Germany)
|August 20, 2024
概括
架构球体的混沌辅助生产 (CAPAS) 实现了用于组织工程的多层水凝球体的快速,高吞吐量生成. 这种多功能方法保持了高细胞活力,为药物测试创造了复杂的微利基.
科学领域:
- 生物材料科学 生物材料科学
- 生物制造的生物制造
- 组织工程是组织工程.
背景情况:
- 具有内部隔间的水凝滴对于组织工程等应用至关重要.
- 创建此类结构的现有方法可能很慢,缺乏可扩展性.
研究的目的:
- 介绍一种新的以滴滴为基础的生物制造方法,用于生产建筑球体 (CAPAS).
- 为了实现高通量,快速生成具有受控内部隔间的多层水凝球.
主要方法:
- 利用凯尼克斯静态混合器 (KSM) 喷嘴产生的混乱向导来形成滴滴.
- 通过流速,喷嘴直径,聚合物度 (基酸盐,GelMA) 和交叉连接浴,控制球体大小.
- 在滴水和喷射模式中都经过证明的操作,保留了多层架构.
主要成果:
- 成功制造了高速率 (高达2000/分钟) 的多层水凝球 (0.63.5毫米直径).
- 在制造后立即和经过延长培养后,为乳腺癌,纤维细胞和肌细胞细胞系实现了超过80%的细胞活力.
- 创建了一个乳腺癌模型,用于药物疗效测试,具有独特的微利基.
结论:
- CAPAS是一种多功能和高效的方法,用于制造复杂的水凝球.
- 该技术支持用于组织工程,化学工程和材料科学中的各种应用的高吞吐量生产.
- 展示了用于制造药物查和疾病建模的体外模型的潜力.
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