Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Chemoproteomics unveils the antibacterial mechanism of silver ions: inhibiting peptidoglycan synthesis <i>via</i> targeting Mur family proteins in <i>Staphylococcus aureus</i>.

Chemical science·2026
Same author

Covalent Inhibition of SHMT2 by Gambogic Acid Induces Ferroptosis Through Mitochondrial Collapse in Triple-Negative Breast Cancer.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Programmable enhancement of endogenous mRNA translation through CRISPR-guided epitranscriptomic regulation.

Cell discovery·2026
Same author

A large-scale plasma proteomic study reveals the preclinical evolution and potential biomarkers for coronary atherosclerosis.

Cardiovascular pathology : the official journal of the Society for Cardiovascular Pathology·2026
Same author

Fexofenadine promotes bone formation and inhibits bone resorption via dual targeting of cPLA2 and Smad2.

International immunopharmacology·2026
Same author

Machine Learning Approaches for Compound-Target Interaction Prediction: A Review.

Foods (Basel, Switzerland)·2026

相关实验视频

Updated: Jun 16, 2025

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays
10:44

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Published on: November 13, 2017

6.5K

方便的两步方法构建了基于的微流体芯片,用于快速检测CYP2C19SNP.

Haobo Wang1, Chi Yan1, Hua Tong1

  • 1School of Materials Science and Engineering, East China University of Science and Technology Shanghai 200237 China liucui@ecust.edu.cn lihongbo@ecust.edu.cn.

RSC advances
|August 20, 2024
PubMed
概括
此摘要是机器生成的。

本研究引入了一种微流体装置,用于快速检测CYP2C19基因单核酸多态 (SNP). 芯片上的定量实时PCR (qPCR) 系统显著减少了个性化克洛皮多格雷尔治疗的测试时间和成本.

更多相关视频

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications
14:43

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications

Published on: September 23, 2013

10.8K
A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

14.9K

相关实验视频

Last Updated: Jun 16, 2025

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays
10:44

Snap Chip for Cross-reactivity-free and Spotter-free Multiplexed Sandwich Immunoassays

Published on: November 13, 2017

6.5K
Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications
14:43

Microfluidic On-chip Capture-cycloaddition Reaction to Reversibly Immobilize Small Molecules or Multi-component Structures for Biosensor Applications

Published on: September 23, 2013

10.8K
A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
15:41

A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells

Published on: October 15, 2013

14.9K

科学领域:

  • 生物技术是生物技术.
  • 分子诊断学 分子诊断学
  • 遗传学 遗传学 是一个

背景情况:

  • 准确检测CYP2C19基因单核酸多态 (SNP) 对于优化克洛皮多格雷尔药物治疗至关重要.
  • 传统的定量实时聚合酶连锁反应 (qPCR) 方法用于SNP检测是耗时和试剂密集的.

研究的目的:

  • 开发一种快速且具有成本效益的微流体装置,用于基于芯片上的qPCR检测CYP2C19SNP.
  • 在速度和资源利用方面改善传统qPCR系统的局限性.

主要方法:

  • 设计和制造具有集成快速热循环和光学检测系统的微流体芯片.
  • 实施芯片上的qPCR测定用于检测CYP2C19SNP.
  • 与商业 qPCR 仪器相比,开发的设备的性能进行了比较.

主要成果:

  • 微流体装置在15分钟内实现了CYP2C19 SNP检测,这与商业仪器的1小时测试时间相比显著减少.
  • 在芯片上的qPCR显示了与商业系统相比较的线性,增强了放大效率.
  • 该设备采用成本高效的两步制造工艺,用于玻璃芯片.

结论:

  • 开发的微流体芯片上的qPCR装置为CYP2C19 SNP检测提供了一个快速,高效和潜在的低成本的解决方案.
  • 这项技术在临床检测 (POCT) 应用中显示出显著的前景,使得克洛皮多格雷尔治疗的临床决策更快.
  • 该设备解决了传统qPCR的局限性,为更容易获得的基因测试铺平了道路.