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Confocal Fluorescence Microscopy

Confocal Fluorescence Microscopy

Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...

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Updated: Jun 16, 2025

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

基于空间复杂干涉测量的脱轴数字无镜头全息显微镜.

José Ángel Picazo-Bueno^1,2, Steffi Ketelhut¹, Jürgen Schnekenburger¹

¹University of Muenster, Biomedical Technology Center, Muenster, Germany.

Journal of biomedical optics

|August 20, 2024

概括

无镜头空间复杂干扰显微镜 (LESSMIM) 为快速移动的生物样本提供了无双图像的定量相位成像. 这种紧的,没有透镜的技术可以在流细胞计和精子分析等领域推进应用.

关键词:

数字全息显微镜的数字全息显微镜.数字无镜头全息显微镜.没有标签的成像技术.离轴无镜头全息学无镜头全息学阶段检索恢复的阶段检索.定量阶段成像成像技术空间复杂干扰计显微镜.

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Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

Published on: February 8, 2014

相关实验视频

Last Updated: Jun 16, 2025

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

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Published on: February 8, 2014

科学领域:

生物医学光学生物医学光学
显微镜的使用方法
定量阶段成像技术定量阶段成像技术

背景情况:

数字全息显微镜 (DHM) 为生物样本提供无标签,时间分辨率的定量相成像 (QPI).
无镜头DHM (DLHM) 提供了紧的,具有成本效益的系统,适用于资源有限的设置.
在线DLHM受双图像文物限制,阻碍了准确的QPI.

研究的目的:

为快速移动的生物标本的QPI开发一个紧的,没有透镜的,通路的干扰度离轴方法.
为了克服直线DLHM的双图像限制.

主要方法:

引入了无透镜空间复杂干扰显微镜 (LESSMIM),这是SMIM的无透镜变体.
采用了一种通路干扰度架构,单一的衍射格用于数字离轴全息.
从单拍全息图中采用福里埃过,偏差补偿和从单拍全息图中获得双图像无QPI的数值传播.

主要成果:

用分辨率测试图表展示了 LESSMIM 概念,并评估了时间稳定性.
标志着QPI准确度和对活体附着细胞培养的成像能力.
使用微流体通道对流动中的悬浮细胞进行评估细胞计量.

结论:

LESSMIM在一个紧的光学设置中提供快速,时间解析的QPI,克服了线内DLHM的局限性.
该技术对需要高速成像的生物医学应用具有前景,例如成像流细胞计和精子细胞分析.