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相关概念视频

siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

16.7K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
16.7K
RNA Interference01:23

RNA Interference

26.0K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.0K
Types of RNA01:20

Types of RNA

5.7K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
5.7K
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

7.6K
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
7.6K
Experimental RNAi02:15

Experimental RNAi

6.1K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.1K
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

5.6K
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
5.6K

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相关实验视频

Updated: Jun 16, 2025

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes
11:58

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes

Published on: January 30, 2019

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小分子RNA降解剂

Javier Bonet-Aleta1, Tomoaki Maehara1, Benjamin A Craig1

  • 1Yusuf Hamied Department of Chemistry, University of Cambridge, CB2 1EW, Cambridge, United Kingdom.

Angewandte Chemie (International ed. in English)
|August 20, 2024
PubMed
概括

将简单的RNA结合剂重新编程成强大的RNA降解剂对于推进RNA向疗法至关重要. 本综述探讨了将RNA结合剂转化为有效降解剂的策略,从而增强治疗潜力.

科学领域:

  • 分子生物学分子生物学
  • 药用化学 医学化学
  • 药物发现 药物发现 药物发现

背景情况:

  • RNA在生物过程和疾病中至关重要,使其成为治疗点.
  • 目前的RNA向策略经常面临由于非功能性RNA相互作用的限制.
  • 小分子与RNA结合并不总是转化为临床疗效.

研究的目的:

  • 审查将小分子RNA结合剂转化为RNA降解剂的策略.
  • 探索增强RNA向治疗功效的机制.
  • 为推进RNA向药物发现提供见解.

主要方法:

  • 关于将RNA结合剂重新编程为降解剂的文献综述.
  • 降解机制的分类:内源酶介导 (RIBOTACs) 和自主降解.
  • 分析涉及功能组与小分子结合剂联系的策略.

主要成果:

  • 小分子结合剂可以被设计成RNA降解剂.
  • 确定了两个主要类型的RNA降解策略:RIBOTACs和自主降解剂.
  • 成功的重编程增强了RNA向剂的治疗潜力.

结论:

关键词:
这是一个RNARNARNARNARNA.降解降解降解降解降解.靠近引起的亲近感.小分子分子的小分子.治疗疗法治疗疗法治疗疗法

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A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
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A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

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Improving Small RNA-seq: Less Bias and Better Detection of 2'-O-Methyl RNAs
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Improving Small RNA-seq: Less Bias and Better Detection of 2'-O-Methyl RNAs

Published on: September 16, 2019

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相关实验视频

Last Updated: Jun 16, 2025

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes
11:58

Using In Vitro and In-cell SHAPE to Investigate Small Molecule Induced Pre-mRNA Structural Changes

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A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools
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A Complete Pipeline for Isolating and Sequencing MicroRNAs, and Analyzing Them Using Open Source Tools

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Improving Small RNA-seq: Less Bias and Better Detection of 2'-O-Methyl RNAs
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Improving Small RNA-seq: Less Bias and Better Detection of 2'-O-Methyl RNAs

Published on: September 16, 2019

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  • 将RNA结合剂转化为降解剂提供了一个有前途的治疗策略.
  • 工程RNA降解剂可以克服简单结合剂的局限性.
  • 这一领域的进一步发展具有治疗疾病的巨大潜力.