通过UHPLC-Q Exactive HRMS对各种水果和蔬菜进行多种农药残留物查,识别和量化分析
Fengyi Wang1,2, Ying Jiao1,2, Shiting Qiu1,2
1Institute of Quality Standard and Testing Technology for Agro-products of Sichuan Academy of Agricultural Sciences, Chengdu 610066, China. SCNKYHM@163.com.
Analytical methods : advancing methods and applications
|August 20, 2024
概括
一种新方法使用超高性能液态色谱-高分辨率质谱仪准确检测水果和蔬菜中的400多种农药. 这种经过验证的方法通过提供可靠的农药残留分析来确保食品安全.
科学领域:
- 分析化学 分析化学
- 食品科学 食品科学 食品科学
- 环境科学 环境科学
背景情况:
- 对食品中的农药残留物分析对于公共卫生至关重要.
- 对于各种食物矩阵,需要准确和灵敏的检测方法.
研究的目的:
- 开发和验证403种农药的定性查和定量分析的综合方法.
- 通过监测农药残留物来确保水果和蔬菜的安全.
主要方法:
- 超高性能液态染色学 (UHPLC) 与高分辨率质谱学 (HRMS) 相结合.
- 利用一个可执行的复合数据库,提供精确的质量,保留时间和碎片离子.
- 轨道探测分析仪用于高灵敏度全扫描和MS/MS模式.
主要成果:
- 选择性识别8个同位素对和57个同位素化合物组.
- 对于68.0-84.4%的农药,查检测极限 (SDL) ≤10μgkg-1
- 在强化水平下,回收率在60-120%之间,精度在20%以下;平均回收率为82.4-105.1%,RSD为3.9-7.7%.
结论:
- 开发的UHPLC-HRMS方法对各种水果和蔬菜中的敏感和选择性农药残留分析是有效的.
- 经过验证的方法为食品安全检查和监督提供可靠的数据.
- 这种方法支持监管合规和关于农药暴露的消费者保护.
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