相关实验视频
Updated: Jun 16, 2025

13:10
Use of Alu Element Containing Minigenes to Analyze Circular RNAs
Published on: March 10, 2020
7.3K
通过一种新的 cis-splicing 系统有效地循环化编码蛋白质的 RNA
Shaojun Qi1, Huiming Wang1, Guopeng Liu1
1Department of mRNA Sciences, Suzhou Abogen Biosciences Co., Ltd., Suzhou 215123, China.
Nucleic acids research
|August 20, 2024
概括
这项研究提出了一种新的cis-splicing系统,用于高效的循环RNA (circRNA) 生产. 该工程系统增强蛋白质表达并减少免疫反应,推进circRNA疗法.
科学领域:
- 分子生物学分子生物学
- 在RNA治疗方面,RNA疗法.
- 生物技术是生物技术.
背景情况:
- 循环RNAs (circRNAs) 在治疗应用中比线性mRNA具有独特的优势.
- 有效和可控的circRNAs生产对于它们的发展至关重要.
- 现有的circRNA合成方法往往需要复杂的设计或额外的元素.
研究的目的:
- 为 circRNA 生产开发一种新且高效的 cis-splicing 系统.
- 调查有效的circRNA自我拼接和循环化的结构要求.
- 在蛋白质表达和免疫性方面评估产生的circRNAs的性能.
主要方法:
- 引入一个 ribozyme 核心进入前体RNA 进行 cis-splicing.
- 同类臂的设计,以形成P9.0双重组,用于自拼接.
- 结构优化RNA元素 (P10,P1-ex) 以提高循环化.
- 对circRNA产生,蛋白质表达和先天免疫反应的分析.
主要成果:
- 该 cis-splicing 系统有效地产生 circRNAs 没有额外的间隔元件.
- 形成P9.0双重组对于自剪接和循环化至关重要.
- 与 ωG 位置相邻的结构特征,而不仅仅是核酸组成,决定了对转化部位的识别.
- 优化的结构元素显著提高了循环化效率.
- 生成的circRNAs显示蛋白质表达延长和低天生的免疫激活.
结论:
- 开发的cis-splicing系统为circRNA生成提供了一个有效的策略.
- 了解RNA结构元素是优化circRNA合成的关键.
- 这种方法有望推进基于circRNA的疗法,以提高稳定性和降低免疫性.
相关概念视频
RNA Splicing
56.2K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
56.2K
Alternative RNA Splicing
21.1K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
21.1K
Pre-mRNA Processing: RNA Splicing
5.2K
5.2K
Experimental RNAi
6.1K
RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
6.1K
RNA Interference
26.0K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
26.0K
piRNA - Piwi-interacting RNAs
6.8K
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
6.8K

