鉴定和基因组分析一种热友细菌菌株,该菌株可以减少堆肥造成的氨损失
Xuejuan Chen1, Rong Feng2, Qianhui Du1
1College of Agriculture, Guizhou University, Guiyang, Guizhou, China.
Microbiology spectrum
|August 20, 2024
概括
一种新的热友细菌Priestia aryabhatta LL-8在高温有氧堆肥过程中有效地减少了氨的损失. 这种吸收氨的菌株通过转化挥发性氨来改善堆肥质量并减轻空气污染.
科学领域:
- 微生物学 微生物学
- 环境科学 环境科学
- 生物技术是生物技术.
背景情况:
- 氧化堆肥对于有机废物回收利用至关重要,但会遭受严重的氨挥发,特别是在高温 (>50°C) 时.
- 这种氨损失导致堆肥中的大量耗尽,并导致空气污染.
- 利用热友微生物在高温阶段对氨进行同化提供了一个潜在的解决方案.
研究的目的:
- 选和识别热友,吸收氨的细菌,能够减少在有氧堆肥过程中的氨损失.
- 评估所选菌株在便堆肥中减少氨挥发的有效性.
- 阐明鉴定出的菌株氨同化能力的基因组基础.
主要方法:
- 对高温氨同化细菌菌株的查.
- 在肥堆肥中应用所选菌株 (LL-8),以评估减少氨损失.
- 植物遗传学分析用于菌株识别和全基因组测序.
- 生物信息分析 (KEGG数据库) 来识别与氨同化相关的基因.
主要成果:
- 一种被称为Priestia aryabhatta LL-8的细菌菌株在50°C时表现出高氨转化率 (32.7%).
- 使用P. aryabhatta LL-8在堆肥的高温阶段显著减少了42.9%的氨挥发性损失.
- 基因组分析揭示了关键基因 (例如,gudB,rocG,glnA,gltA,gltB) 的存在,这些基因参与了氨同化.
结论:
- Priestia aryabhatta LL-8是一种有前途的热友,吸收氨的细菌,用于减轻有氧堆肥中氨的损失.
- 这种菌株的应用可以提高堆肥中的含量,并减少相关的空气污染.
- 这些基因组洞察力为P. aryabhatta LL-8在堆肥过程中的有针对性的应用提供了基础.
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