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Updated: Jun 24, 2026

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有效和快速的一步方法,以产生Streptococcus pyogenes中的基因删除

Lionel Schiavolin1, Dalila Lakhloufi1, Gwenaelle Botquin1

  • 1Molecular Bacteriology, European Plotkin Institute for Vaccinology (EPIV), Université libre de Bruxelles, Brussels, Belgium.

Microbiology spectrum
|August 20, 2024
PubMed
概括

一种新方法可以快速生成A组链球菌 (GAS) 突变体,克服疫苗开发和对这一重要的全球病原体的毒性因子研究的挑战.

关键词:
这是一种Streptococcus pyogenes.淘汰赛 淘汰赛 是一个淘汰赛.快速方法快速方法.

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DNA Isolation01:24

DNA Isolation

DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...