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在Cordyceps militaris中的核心微生物和它们与代谢相关的生态功能

Li Luo¹, Fei Dai², Zhongshun Xu¹

¹Institute of Fungus Resources, College of Life Science, Guizhou University, Guiyang, Guizhou, China.

Microbiology spectrum

|August 20, 2024

概括

在Cordyceps militaris sclerotia中的核心微生物影响其生长和代谢物生产. 细菌将酸盐转化为,酸盐对于cordycepin合成至关重要,揭示了它对代谢调节的关键作用.

科学领域:

微生物生态学微生物生态学
菌类学菌类学是指菌类学.
生物化学生物化学

背景情况:

科尔迪塞普斯军用菌在它的硬化菌中藏着共生微生物.
众所周知,这些微生物会影响宿主真菌的生长和代谢物生产.
了解这些相互作用对于优化生产有价值的化合物至关重要.

研究的目的:

在五年时间内,在Cordyceps militaris sclerotia中确定稳定的核心微生物群落.
调查与Cordyceps militaris代谢物生产和代谢有关的孤立细菌菌株的功能作用.
阐明细菌通过循环影响Cordyceps militaris代谢物合成的间接机制.

主要方法:

在五年内收集的硬化菌样本的高通量DNA测序.
从硬化菌中分离并对7种细菌菌株进行生物化学表征.
与Cordyceps militaris一起共同培养细菌的实验.
在不同基介质 (NH4,NO3,) 中对Cordyceps militaris生长和代谢产物的比较分析.
用RNA测序和实时光定量PCR进行基因表达分析.

主要成果:

包括Rhodococcus和Pseudomonas在内的9个细菌属被确定为核心微生物.
孤立的细菌表现出酸盐降解和尿素分解能力.
在NH4介质中培养Cordyceps militaris与NO3或子介质相比,显著增加了cordycepin水平.
细菌被证明可以将NO3转化为NH4,这一过程与增强的皮质素生产有关.

结论:

科尔迪塞普斯军方性硬化菌 (Cordyceps militaris sclerotia) 拥有稳定的核心微生物群落.
这些细菌在昆虫微生物复合体内调节代谢方面发挥着重要作用.
细菌对代谢的调节,特别是酸盐转化为,间接增强Cordyceps militaris中的皮质素生产.

关键词:

科尔迪塞普斯军用菌 (Cordyceps militaris) 是一种植物.社区集会社区集会科尔迪斯皮恩是什么意思核心的微生物群.微生物的功能.的新陈代谢的新陈代谢

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