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深度分支的Bacillota物种表现出非典型的格拉姆阴性染色

Jessica K Choi¹, Saroj Poudel², Nathan Yee^3,4

¹Ecology and Evolutionary Biology Department, University of Michigan, Ann Arbor, Michigan, USA.

Microbiology spectrum

|August 20, 2024

概括

格拉姆染色方法可能并不总是反映细菌细胞壁结构. 一些具有单一膜 (单体) 的细菌可以染色格兰氏阴性,挑战传统的解释,并揭示细胞壁发育的进化见解.

科学领域:

微生物学微生物学
进化生物学进化生物学
细菌细胞信封研究研究

背景情况:

格拉姆染色传统上根据单体 (格拉姆阳性) 和二体 (格拉姆阴性) 细胞包裹区分细菌.
类Bacillota表现出多样化的细胞壁类型,单皮动物从多皮动物祖先进化了多次.

研究的目的:

为了研究在Bacillota族群内Gram-阳性和Gram-阴性细胞壁类型的进化.
重新评估Gram染色结果与细菌细胞外超结构之间的相关性.

主要方法:

用完整基因组编制的Bacillota物种的Gram染色和超结构数据的汇编.
遗传学重建以推断进化关系和细胞壁类型起源.

主要成果:

许多深度分支的Bacillota血统染色为Gram负,但缺乏外膜生物合成基因.
遗传学分析表明,在一些格拉姆阴性染色物种中,体祖先保留了一层薄薄的酸甘层.
确定了非典型的格兰氏阴性染色种群,包括Symbiobacterium thermophilum和Desulfotomaculia,Syntrophamonadia,Desulfitobacteriia,Thermosediminibacteria和Thermoanaerobacteria的成员.

结论:

格拉姆阴性染色并不仅仅表明所有细菌中的二体细胞结构.
格拉姆阳性细胞包裹的进化可能涉及二甲糖加厚的多个独立事件.

关键词:

格拉姆阴性细菌是一种细菌.格拉姆阳性细菌是什么?细胞壁上的细胞壁.进化演化演化演化演化演化演化演化基因组学就是基因组学.

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