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通过Bacillus amyloliquefaciens增强合成S-adenosylmethionine的多层次代谢工程

Cong Jiang¹, Dian Zou¹, Liying Ruan¹

¹State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, No. 1 Shizishan Street, Hongshan District, Wuhan, 430070, Hubei, People's Republic of China.

Biotechnology letters

|August 20, 2024

概括

基因工程Bacillus amyloliquefaciens的基因工程增强了S-adenosylmethionine (SAM) 的生产. 删除 argG 基因和过度表达 SAM2 基因显著提高了 SAM 产量,达到 452.89 mg/L 的最大标位.

科学领域:

微生物学微生物学
代谢工程是代谢工程.
合成生物学合成生物学

背景情况:

S-adenosylmethionine (SAM) 是一个关键的代谢物,参与了许多细胞甲基化反应.
在微生物宿主中增强新的SAM合成对于生物技术应用至关重要.
氨酸菌是有价值化合物的工业生产的一个有前途的平台.

研究的目的:

为了改善SAM在Bacillus amyloliquefaciens中的新合成.
通过模块化策略识别和操纵影响SAM生产的关键基因.
为了提高产量,设计一种高SAM产生的菌株.

主要方法:

利用模块化策略来检查关键基因对SAM合成的影响.
采用基因删除 (argG, moaA) 和基因过度表达 (citA, SAM2) 技术.
构建了Bacillus amyloliquefaciens的工程菌株,以提高SAM的生产.

主要成果:

删除 argG 基因增加了SAM 标位18%,达到254.78 mg/L.
删除 moaA 基因会影响生长,降低 SAM 产量.
使用多拷贝等离子体的SAM2基因过度表达导致了最高的SAM标位 (452.89毫克/升).

结论:

删除 argG 基因有效地阻止了酸分支降解,增强了 SAM 生产.
使用多拷贝等离子体对SAM2基因的过度表达是显著改善SAM合成的最有效策略.
改造的Bacillus amyloliquefaciens菌株显示了工业SAM生产的巨大潜力.

关键词:

亚米洛基面菌 (Bacillus amyloliquefaciens) 是一种有毒的植物.代谢工程是代谢工程.模块化战略是一个模块化战略.这是一种S-adenosylmethionine.

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