免疫接种后对抗原特异性B细胞受体的计算检测
Maria Francesca Abbate1,2, Thomas Dupic3, Emmanuelle Vigne2
1Laboratoire de physique de l'École normale supérieure, CNRS, Paris Sciences et Lettres University, Sorbonne Université, and Université Paris-Cité, Paris 75005, France.
我们开发了一种计算方法,从免疫谱中识别抗原特异性B细胞受体 (BCRs). 这种方法可以准确地检测响应的BCR序列,有助于理解免疫反应和开发新疗法.
科学领域:
- 免疫学 免疫学 免疫学
- 生物信息学是一种生物信息学.
- 计算生物学 计算生物学
背景情况:
- B细胞受体 (BCRs) 是识别抗原的关键,但在大范围范围内识别特定的响应者是具有挑战性的.
- 高通量测序提供了对免疫接种后BCR谱的深入洞察.
- 在感染期间,只有很小的一小部分BCRs积极响应.
研究的目的:
- 开发和验证一种计算方法,从免疫谱数据中准确识别抗原特异性B细胞受体序列.
- 根据序列相似性和免疫反应特征,评估该方法检测响应的BCR的能力.
主要方法:
- 一种新的计算方法,使用序列相似性来识别统计学上显著的响应性BCR.
- 利用亲和力成熟和预期多样性的原则进行序列分析.
- 使用从流感疫苗接种和SARS-CoV-2感染的纵向人类免疫谱系数据进行验证.
主要成果:
- 该方法成功地确定了统计学上显著的抗原特异性BCR序列.
- 证明了收选择,不同的B细胞系在相同的互补性确定区域3 (CDR3) 序列上收.
- 使用真实世界的人类免疫反应数据进行有效的验证.
结论:
- 开发的计算方法准确地从目录数据中识别出抗原特异性BCR.
- CDR3序列的融合选择突出显示了个体内保存的免疫反应机制.
- 该方法在疫苗开发,个性化医学和抗体治疗方面具有重大潜力.
更多相关视频
00:09Single-cell Screening Method for the Selection and Recovery of Antibodies with Desired Specificities from Enriched Human Memory B Cell Populations
Published on: August 22, 2019
08:26The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity
Published on: December 14, 2016
相关概念视频
Diversity of Antigen Receptors
Before encountering any antigen, lymphocytes express these receptors. On B cells, the antigen receptor is a membrane-bound antibody molecule called BCR; on T cells, it is a T cell receptor or TCR. B and T cell receptors are composed of two...
B Cell Activation and Differentiation
When naive B cells encounter a specific antigen that can bind to the B cell receptor (BCR) on their surface, they undergo sensitization to respond to the antigen's presence. Sensitization begins with...
Humoral Immune Responses
Special Features of Adaptive Immunity
The primary cell types involved in adaptive immunity are T cells and B cells. Each type has a unique role in defending the body against pathogens. T cells are responsible for cell-mediated immunity. They identify and eliminate infected cells directly,...
Immunological Memory
What is Immunological Memory?
Immunological memory is an integral function of the immune system that allows it to recognize and react more rapidly and effectively to pathogens previously encountered. This feature...
Vaccinations
