Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RNA-seq03:21

RNA-seq

9.9K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.9K
Comparing Copy Number Variations and SNPs02:26

Comparing Copy Number Variations and SNPs

17.7K
Sequencing of the human genome has opened up several best-kept secrets of the genome. Scientists have identified thousands of genome variations that exist within a population. These variations can be a single nucleotide or a larger chromosomal variation.
Copy number variations or CNVs are the structural variations that cover more than 1kb of DNA sequence. The single nucleotide polymorphism (SNP), on the other hand, is a single nucleotide change or a point mutation that is found in more than 1%...
17.7K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Gene-Specific Endothelial Programs Drive AVM Pathogenesis in <i>SMAD4</i> and <i>ALK1</i> Loss-of-Function.

Arteriosclerosis, thrombosis, and vascular biology·2026
Same author

Lentiviral single-cell MPRA of synthetic enhancers reveals motif affinity-based encoding of cell state specificity.

Genome biology·2026
Same author

Microglial TDP-43 mediates myelin refinement and represses Tyrobp cryptic exon inclusion in mice.

Nature neuroscience·2026
Same author

circVDJ-seq for T cell clonotype detection in single-cell and spatial multi-omics.

Genome medicine·2026
Same author

HDAC7 controls anti-viral and anti-tumor immunity by CD8<sup>+</sup> T cells.

Frontiers in immunology·2026
Same author

Non-Temperature-Induced Antitumor Effects of Amplitude-Modulated Radiofrequency: Molecular and Functional Synergies with Radiotherapy.

Cancers·2026

相关实验视频

Updated: Jun 16, 2025

Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing
11:02

Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing

Published on: October 18, 2013

19.4K

在scRNA-seq数据中从调用单核酸变异中识别癌细胞.

Valérie Marot-Lassauzaie1,2, Sergi Beneyto-Calabuig3,4, Benedikt Obermayer5

  • 1Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin Institute for Medical Systems Biology (BIMSB), Hannoversche Str. 28, 10115 Berlin, Germany.

Bioinformatics (Oxford, England)
|August 20, 2024
PubMed
概括

我们开发了CCLONE,这是一种通过分析杂的遗传变异来识别单细胞RNA测序数据中的癌细胞的工具. CCLONE精确识别癌症克隆及其突变,提供有关癌症起源和疾病进展的见解.

更多相关视频

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
11:52

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

10.3K
Rare Event Detection Using Error-corrected DNA and RNA Sequencing
10:36

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Published on: August 3, 2018

12.0K

相关实验视频

Last Updated: Jun 16, 2025

Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing
11:02

Detecting Somatic Genetic Alterations in Tumor Specimens by Exon Capture and Massively Parallel Sequencing

Published on: October 18, 2013

19.4K
Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations
11:52

Targeted RNA Sequencing Assay to Characterize Gene Expression and Genomic Alterations

Published on: August 4, 2016

10.3K
Rare Event Detection Using Error-corrected DNA and RNA Sequencing
10:36

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Published on: August 3, 2018

12.0K

科学领域:

  • 基因组学就是基因组学.
  • 生物信息学是一种生物信息学.
  • 癌症研究 癌症研究

背景情况:

  • 单细胞RNA测序 (scRNA-seq) 对于研究癌细胞异质性至关重要.
  • 在scRNA-seq数据中区分瘤细胞和健康细胞是具有挑战性的,因为人口混合.
  • 实体单核酸变体 (SNVs) 可以识别癌细胞,但很难从scRNA-seq数据中准确地调用.

研究的目的:

  • 开发一种可解释的工具,CCLONE,用于从scRNA-seq数据中使用SNV识别癌细胞群.
  • 为应对scRNA-seq.中的杂和稀疏的SNV数据所带来的挑战.
  • 共同识别癌症克隆及其相关变体.

主要方法:

  • 开发了CCLONE (癌细胞对噪声表达的标记),这是一个针对噪声和稀疏SNV数据设计的算法.
  • CCLONE集成了变体调用与克隆群体识别.
  • 该工具适用于处理scRNA-seq数据中固有的不确定性.

主要成果:

  • 在多个患者数据集中,CCLONE成功识别了基因克隆和体征事件,包括急性髓性白血病和肺腺癌.
  • 该工具展示了其捕获复杂克隆结构的能力.
  • 结果强调了CCLONE在分析用于癌症研究的scRNA-seq数据方面的有效性.

结论:

  • CCLONE提供了一种强大的方法,用于在异质瘤微环境中识别癌细胞及其遗传构成.
  • 该工具提供了有关癌细胞起源和疾病进展的宝贵见解.
  • CCLONE增强了scRNA-seq数据对于癌症基因组学研究的实用性.