FOXM1通过调节老化卵细胞中的p21来影响氧化应激,线粒体功能和DNA损伤反应
Wenjie Yu1, Xiaoshi Cai1, Chen Wang1
1College of Animal Sciences, Jilin University, Changchun, 130062, Jilin, China.
Theriogenology
|August 20, 2024
概括
叉头盒M1 (FOXM1) 通过调节氧化应激,DNA损伤和衰老,在猪卵细胞衰老中发挥着关键作用. 抑制FOXM1会使衰老恶化,而抑制p21会减轻这些影响,从而提供对卵细胞保存的见解.
科学领域:
- 生殖生物学 生殖生物学
- 分子遗传学 分子遗传学
- 细胞衰老 细胞衰老
背景情况:
- 卵细胞的排卵后衰老减少了受精和胚胎存活率,影响了生殖率.
- 驱动卵细胞衰老的关键调节基因和机制在很大程度上是未知的.
- 叉头盒M1 (FOXM1) 涉及与衰老相关的细胞过程,包括氧化应激和衰老.
研究的目的:
- 研究FOXM1在猪卵细胞衰老中的作用和潜在机制.
- 检查FOXM1在卵细胞衰老期间对氧化应激,线粒体功能,DNA损伤和亡的影响.
主要方法:
- RNA测序 (RNA-Seq) 用于识别老化的猪卵细胞中差异表达的基因.
- 试验操纵卵细胞中的FOXM1和p21水平.
- 测试用于测量活性氧物种 (ROS),抗氧化剂水平 (GSH,SOD,T-AOC,CAT),DNA损伤,细胞亡和细胞衰老.
主要成果:
- RNA-Seq鉴定了3237个差异表达的基因,在老化卵细胞中增加了FOXM1的表达.
- 抑制FOXM1会加剧氧化应激,DNA损伤,细胞亡和老化的卵细胞.
- 抑制p21部分挽救了FOXM1抑制对卵细胞衰老标志物的负面影响.
结论:
- FOXM1是猪卵细胞衰老的关键调节者,影响关键的细胞过程.
- 福克斯M1的保护作用包括管理氧化应激和DNA完整性.
- 这些发现为预防卵细胞衰老和改善体外培养条件的策略提供了基础.
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