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胚胎热操纵改变了化后的子的发育和肝脂代谢
Sheng Li1, Yuyan Wang1, Siyu Guo1
1Research Centre for Livestock Environmental Control and Smart Production, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, China.
Journal of animal science
|August 20, 2024
概括
胚胎热操纵 (TM) 影响层的发育. 高温 (TMH) 加快了化,而低温 (TML) 降低了化能力和改变了肝脂代谢,随着时间的推移,效应会减弱.
科学领域:
- 动物科学动物科学
- 胚胎学 胚胎学
- 代谢生理学 代谢生理学
背景情况:
- 化温度极大地影响胚胎发育和家禽的质量.
- 肝脏脂质代谢对于家禽的生长和发育至关重要.
- 了解热操纵对胚胎发育和新陈代谢的影响对于优化家禽生产至关重要.
研究的目的:
- 为了研究高 (TMH) 和低 (TML) 胚胎热操纵对化性能和层的肝脂代谢的影响.
- 通过短期养试验,评估TM对生长和新陈代谢的影响的持续时间.
主要方法:
- 在第八到第十五个胚胎日之间,层胚胎接受了TMH (39.5°C,8小时/天) 或TML (20°C,1小时/天).
- 评估了化性能,生长,残留黄,相对肝脏重量和肝/血清脂质含量 (三糖,总胆固醇).
- 分析了肝脂滴积累 (Oil Red O染色),基因表达 (脂生/氧化) 和酶活性 (LPL).
主要成果:
- TMH加速了化;TML延迟了化,减少了化能力,降低了小的质量,增加了剩余的蛋黄和减少了相对肝脏重量.
- TMH没有显著改变肝/血清脂质,但上调了脂质生成基因 (ACC,Fas,Fatp1).
- TML降低了肝脏甘油三,增加了LPL活性,并促进了脂质氧化基因 (CPT-1,PGC-1α,PPARα);TMH在7天后增加了LPL活性,但TML对脂质和基因表达的影响在TML小中减少了.
结论:
- 胚胎热操纵显著改变化性能和层的肝脂代谢.
- TML增强了脂质氧化能力,降低了甘油三含量,但这些代谢适应是暂时的.
- 胚胎热操纵对层的发育和新陈代谢的长期影响随着的成熟而受到限制.
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