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Updated: Jun 16, 2025

Generating Transposon Insertion Libraries in Gram-Negative Bacteria for High-Throughput Sequencing
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H-NS是一种细菌的转子子捕获蛋白

Charles Cooper1, Simon Legood1, Rachel L Wheat1

  • 1School of Biosciences, University of Birmingham, Birmingham, UK.

Nature communications
|August 20, 2024
PubMed
概括

基因组状核状结构 (H-NS) 蛋白质指导细菌DNA转移到特定区域,防止基本的基因破坏. 这种机制通过影响病原性岛屿来促进临床相关的表型多样性.

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DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
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