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Luigi Coppola1, Anna Maria Grimaldi2, Ginevra Sarnacchiaro2
1IRCCS SYNLAB SDN, Via G. Ferraris 144, Napoli, Italy. luigi.coppola@synlab.it.
Scientific reports
|August 20, 2024
概括
生物库保存高质量的人类样本用于研究. 质量控制测定证实了冷保存的外周血液单核细胞 (PBMC) 和血样本符合翻译研究的标准.
科学领域:
- 这是生物银行.
- 翻译研究是翻译研究.
- 质量控制 质量控制
背景情况:
- 生物库对于存储用于研究的高质量生物样本至关重要.
- 这些样本支持翻译研究和未来疗法的开发.
- 在IRCCS Synlab SDN生物库中,存储了来自各种疾病的约7万个冷保存的人体样本.
研究的目的:
- 建议对生物库样本进行质量控制试验.
- 确保符合ISO 9001:2015和ISO 20387:2020认证标准的要求.
- 评估冷保存的人体样本的质量.
主要方法:
- 通过流式细胞计量评估了外周血液单核细胞 (PBMC) 的活力和免疫亚群细胞.
- 分析了无处不在的microRNA (miRNA) 表达,以确定血样本的完整性.
- 对解生物库样本的评估质量控制指标.
主要成果:
- 冷保存的PBMCs显示出80%以上的活力,适合用于免疫细胞研究.
- 在解的血样本中,miRNA表达显著更高,表明完整性.
- 质量控制试验证实,样品冷保存不会影响样品质量.
结论:
- 生物库的冷保存的PBMC和血样本符合生物医学研究的高质量标准.
- 已建立的质量控制方法确保生物样本的完整性和长期保存.
- 需要进一步的研究来规范全球生物银行质量评估程序.
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