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基因组序列和注释草案的polyextremotolerant聚脂产生的真菌 aureobasidium pullulans NRRL 6204242的注释

Marie R E Dielentheis-Frenken¹, Daniel Wibberg^2,3, Lars M Blank¹

¹Institute of Applied Microbiology, RWTH Aachen University, Aachen, Germany.

BMC genomic data

|August 20, 2024

概括

我们对Aureobasidium pullulans NRRL 62042的基因组进行了测序和注释. 这为了解其在生物技术中的潜力提供了宝贵的资源,特别是用于生产多脂.

科学领域:

微生物学微生物学
基因组学就是基因组学.
生物技术是生物技术.

背景情况:

奥里奥巴西迪亚是一种类似酵母菌的真菌,以生产宝贵的二次代谢物,如多脂等而闻名.
它的多极耐药性和代谢能力使其成为生物技术应用的有希望的候选人.
最近测序技术的进步使得Aureobasidium菌株的详细基因组研究成为可能.

研究的目的:

为提供Aureobasidium pullulansNRRL 62042.2.的注释性基因组序列草案.
为了促进进一步研究这种菌株的生物技术潜力.
支持Aureobasidium的发展,作为有价值化合物的生产宿主.

主要方法:

使用Illumina NovaSeq 6000进行全基因组测序.
基因组组装成39个支架. 基因组组装成39个支架.
使用Genemark v4.68和GenDBE.使用基因注释.

主要成果:

确定了一个24.2 Mb的基因组大小草案.
该基因组的GC含量为50.1%.
总共预测和注释了9596个基因.

结论:

现在可以获得A. pullulansNRRL 62042的注释基因组序列.
这种基因组资源将有助于理解和增强其多脂生产.
这项研究支持将Aureobasidium作为工业生物技术的强大宿主.

关键词:

乌雷奥巴西迪亚姆 (Aureobasidium Pullulans) 是一个有毒的植物.多种极端耐药的多种类型聚胺酸是什么? 聚胺酸是什么聚合物脂质是多聚合物的脂质.普卢卢兰 (Pullulan) 是一个古老的语言.

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Genome Annotation and Assembly

Genome Annotation and Assembly

The genome refers to all of the genetic material in an organism. It can range from a few million base pairs in microbial cells to several billion base pairs in many eukaryotic organisms. Genome assembly refers to the process of taking the DNA sequencing data and putting it all back together in a correct order to create a close representation of the original genome. This is followed by the identification of functional elements on the newly assembled genome, a process called genome annotation.

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Genome Size and the Evolution of New Genes

While every living organism has a genome of some kind (be it RNA, or DNA), there is considerable variation in the sizes of these blueprints. One major factor that impacts genome size is whether the organism is prokaryotic or eukaryotic. In prokaryotes, the genome contains little to no non-coding sequence, such that genes are tightly clustered in groups or operons sequentially along the chromosome. Conversely, the genes in eukaryotes are punctuated by long stretches of non-coding sequence.