外围神经元的功能性表征和一个受体识别性激素在Hyphantria cunea (Erebidae) 中
Guiqiu Di1,2, Zicong Li1,2, Weichao Ma1,2
1School of Forestry, Northeast Forestry University, Harbin 150040, P. R. China.
Journal of agricultural and food chemistry
|August 21, 2024
概括
科学家们确定了秋季网虫如何识别性激素. 素受体HcunPR1a特别识别了一个关键成分,为新的生物杀虫剂提供了一个目标,以控制这种侵入性害虫.
科学领域:
- 昆虫学 昆虫学是一门学科.
- 神经科学是一个神经科学.
- 虫害管理 虫害管理 虫害管理
背景情况:
- 秋季网虫 (Hyphantria cunea) 是一种全球侵袭性害虫,难以控制且成本高昂.
- 性激素捕获是一种有效的监测和管理H. cunea*种群的方法.
- 在H. cunea*中认知性费洛蒙的精确神经机制在很大程度上是未知的.
研究的目的:
- 为了研究Hyphantria cunea*中性激素识别的外围神经机制.
- 为了确定特定的嗅觉神经元和涉及检测性激素的费洛蒙受体基因.
- 为了阐明性激素的神经编码,用于潜在的生物杀虫剂开发.
主要方法:
- 电生理学分析 (单个感觉记录) 来评估对费罗蒙组分的反应.
- 转录组测序以选候选费洛蒙受体 (PR) 基因.
- 基因表达分析以确定PRs在男性和女性天线中的定位.
- 候选PRs的功能识别,以确定它们的激素结合特异性.
主要成果:
- 雄性和雌性 *H. cunea* 都对四个性爱激素成分做出了反应,雄性表现出更强烈的反应.
- 三种类型的三角形感官 (ST) 被确定为对性激素的反应,特别是识别1,Z3,Z6-9S,10R-epoxy-21Hy.
- 确定了四个候选PR基因 (*HcunPR1a*, *HcunPR1b*, *HcunPR3*, *HcunPR4*); *HcunPR1a*的功能已被证实对一个性激素组件有反应.
- 参与性激素识别的神经元位于ST,HcunPR1a识别了二次性激素组成部分1,Z3,Z6-9S,10R-epoxy-21Hy.
结论:
- 这项研究确定了参与 *H. cunea* 性爱激素检测的特定神经元和感官.
- 证实HcunPR1a能够识别关键的性激素成分,从而提供了对外围神经编码机制的洞察力.
- 这些发现表明,主要激素成分的PR可能存在于其他嗅觉受体分支中,这需要进一步调查.
- HcunPR1a代表了开发针对秋季网虫的新型,高度特异性的生物杀虫剂的潜在目标.
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