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沉默CirccHIPK3通过增强miR-338-3pp来改善sevoflurane-explore学习和记忆功能障碍和神经损伤

Xiuli Li¹, Xuefei Li², Yinan Liang³

¹Department of Anesthesiology, Pengzhou People's Hospital , No. 255 South Third Ring Road, Pengzhou 611930, China.

Toxicology research

|August 21, 2024

概括

塞沃弗兰 (Sev) 麻醉会损害学习和记忆. 沉默circHIPK3通过增强miR-338-3p表达来缓解Sev诱导的神经毒性和记忆缺陷,提供了一个潜在的治疗标.

科学领域:

神经科学是一个神经科学.
分子生物学分子生物学
麻醉学麻醉学

背景情况:

塞沃弗兰 (Sev) 是一种常见的麻醉剂.
暴露于Sev可以导致神经毒性,损害学习和记忆等认知功能.

研究的目的:

研究循环RNA HIPK3 (circHIPK3) 在赛沃兰诱导的神经毒性的作用.
阐明涉及circHIPK3和microRNA-338-3p (miR-338-3p) 在认知障碍中的潜在机制.

主要方法:

在Sprague-Dawley大鼠和海马神经元细胞中暴露于sevoflurane.
定量实时PCR (RT-qPCR) 用于测量circHIPK3和miR-338-3p水平.
莫里斯水迷宫 (MWM) 测试用于行为评估,ELISA用于炎症因素,CCK-8,流细胞计和ROS测试用于细胞分析.
双 luciferase 记者 (DLR) 和 RNA 免疫沉 (RIP) 测定以确认 circHIPK3-miR-338-3p 相互作用.

主要成果:

在大鼠海马体和神经细胞中,黄暴露增加了circHIPK3和降低了miR-338-3p水平.
沉默circHIPK3改善了Sev诱导的学习和记忆缺陷,减少了炎症,并减轻了细胞损伤 (活力,亡,ROS).
circHIPK3直接与miR-338-3p结合,其对神经毒性的抑制作用取决于miR-338-3p水平.

结论:

circHIPK3在赛沃兰诱导的神经毒性和认知障碍中发挥着关键作用.
沉默circHIPK3可以缓解Sev诱导的神经毒性和记忆缺陷,这表明针对circHIPK3/miR-338-3p轴的治疗策略.

关键词:

西沃氨酸是什么?在 circHIPK3K3中使用.在 miR-338-3p 中使用.

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