通过STARR-seq. 在大麦中识别具有远程调节潜力的高度重复性增强剂
Wanlin Zhou1,2, Haoran Shi2,3, Zhiqiang Wang1,2
1State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Sichuan Agricultural University, Chengdu 611130, China.
Genomics, proteomics & bioinformatics
|August 21, 2024
概括
研究人员在大麦中使用自转录活性调节区域测序 (STARR-seq) 识别了7323种植物增强剂. 超过75%的测试增强剂是有效的,揭示了广泛的功能范围和重复序列在基因调节中的重要性.
科学领域:
- 植物基因组学 植物基因组学
- 分子生物学分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
背景情况:
- 鉴定植物增强剂是具有挑战性的,因为基因组大小和调节元素的变化.
- 增强剂是调节基因转录启动的关键DNA序列.
研究的目的:
- 在大麦基因组中使用自转录活性调节区域测序 (STARR-seq) 识别增强剂.
- 描述大麦增强剂的特性和功能范围.
主要方法:
- 自转录活性调节区域测序 (STARR-seq) 用于在大麦中全基因组增强剂的识别.
- 双露西法酶记者测试被用来验证所选增强剂的活性.
主要成果:
- 在大麦基因组中发现了7323种增强剂.
- 超过75%的随机选择的增强剂显示了验证的活性.
- 大麦增强剂经常包括重复序列 (53.5%),包括可转移元素 (TE).
- 在确定的增强剂上发现了活性 (H3K4me3) 和抑制性 (H3K27me3) 标记.
- 麦增强剂表现出广泛的功能范围 (±100 kb),比大米或玉米更广泛.
结论:
- STARR-seq有效地识别了植物增强剂,即使是在大基因组中,如大麦.
- 重复序列和TEs在大麦基因表达调节中发挥着重要作用.
- 麦增强剂具有独特的特征,包括广泛的功能范围,可能与高基因表达水平有关.
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