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来自猪场微生物群落的细菌膜囊泡,藏和保护各种功能基因,促进横向基因转移

Jinpeng Li¹, Chao Li², Yun Han¹

¹Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province, College of Life Sciences, Sichuan University, Chengdu 610000, China; Key Laboratory of Bio-Resource and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610000, China.

The Science of the total environment

|August 21, 2024

概括

猪场上的细菌膜囊泡 (BMV) 携带抗生素耐药性基因 (ARG) 和毒性基因. 这些BMV促进基因转移,造成重大环境和健康风险.

科学领域:

环境微生物学环境微生物学
分子生物学分子生物学
公共卫生公共卫生

背景情况:

抗生素耐药性 (AMR) 是全球卫生危机,猪场是抗生素耐药性基因 (ARG) 的重要储库.
细菌膜囊泡 (BMV) 越来越多地被认为是水平基因转移的媒介,但它们在猪场AMR传播中的作用尚不清楚.

研究的目的:

调查猪场环境 (便,土壤,便废水) 中的BMV的分布,来源和微生物起源.
为了识别这些BMV携带的ARG和移动遗传元素 (MGE).
在AMR的背景下评估BMVs的基因传输潜力.

主要方法:

从猪场便,土壤和便废水中取样.
使用颗粒大小和度测量对BMVs的表征.
甲基因组测序以识别BMV中的细菌组成,ARG,毒性基因和MGE.
评估BMV DNA保护和水平基因转移能力.

主要成果:

BMVs是丰富的 (10^810^12颗粒/g) 尺寸从20500nm,含有DNA和蛋白质.
这些BMV主要由蛋白质细菌组成 (例如,Pseudomonadaceae,Moraxellaceae,Enterobacteriaceae).
在BMV中存在多种ARG (对14种抗生素的耐药性,特别是多种药物,四环素,氨基醇耐药性基因) 和74,340个毒性基因.
BMV 含有 MGEs,主要是质粒,保护DNA免受降解,并促进横向基因转移,包括 ARGs.

结论:

在养猪场环境中,BMVs广泛存在,并携带大量的ARG和潜在的毒性基因.
这些BMV在AMR通过水平基因转移在环境传播中发挥着至关重要的作用.
这些发现强调了在农业环境中与BMV介导的基因转移相关的潜在健康风险.

关键词:

抗生素耐药性基因是抗生素耐药性的基因.细菌膜囊泡是一种细菌膜囊泡.功能性基因是指功能性的基因.横向基因转移是指水平基因转移.养猪场是养猪场,可以养猪.

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