Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Next-generation Sequencing03:00

Next-generation Sequencing

88.5K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
88.5K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

IL-1α Expands SLC2A1highSPP1highIFNlow Myeloid Cells to Drive Immune Escape and Malignant Transformation of Pre-cancers.

Cancer research·2026
Same author

Association between thyroid hormone T3 and aggression in drug-naïve schizophrenia: a directed acyclic graph analysis.

European archives of psychiatry and clinical neuroscience·2026
Same author

Adipocyte OX40L promotes adipose T cell activation and insulin resistance in obesity.

Experimental & molecular medicine·2026
Same author

The longitudinal relationships between parent-child alienation and depression in adolescents: insights of network analysis.

Child and adolescent psychiatry and mental health·2026
Same author

Single-Cell Transcriptomic Mapping of PD-L1/TLR4 Remodeling Informs Topical Immunoprevention Timing in Skin Carcinogenesis.

bioRxiv : the preprint server for biology·2026
Same author

ResMap: A community resource for systematic mapping of therapy-persistent residual cancer cell dependencies across contexts.

Science advances·2026

相关实验视频

Updated: Jun 15, 2025

Rare Event Detection Using Error-corrected DNA and RNA Sequencing
10:36

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Published on: August 3, 2018

12.0K

通过增量学习和异常检测来适应纳米孔测序基调模型的修改检测.

Ziyuan Wang1, Yinshan Fang2, Ziyang Liu1,3

  • 1Department of Pharmacy Practice and Science, University of Arizona, Tucson, AZ, USA.

Nature communications
|August 21, 2024
PubMed
概括

本研究介绍了一种机器学习管道,用于使用纳米孔测序检测核酸修饰. 该方法可以准确地识别单分子和单核酸分辨率的修饰,即使在复杂的生物样本中也是如此.

更多相关视频

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example
05:45

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

Published on: March 11, 2020

8.7K
Detection of Copy Number Alterations Using Single Cell Sequencing
09:45

Detection of Copy Number Alterations Using Single Cell Sequencing

Published on: February 17, 2017

11.6K

相关实验视频

Last Updated: Jun 15, 2025

Rare Event Detection Using Error-corrected DNA and RNA Sequencing
10:36

Rare Event Detection Using Error-corrected DNA and RNA Sequencing

Published on: August 3, 2018

12.0K
Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example
05:45

Validating Whole Genome Nanopore Sequencing, using Usutu Virus as an Example

Published on: March 11, 2020

8.7K
Detection of Copy Number Alterations Using Single Cell Sequencing
09:45

Detection of Copy Number Alterations Using Single Cell Sequencing

Published on: February 17, 2017

11.6K

科学领域:

  • 基因组学就是基因组学.
  • 生物信息学是一种生物信息学.
  • 分子生物学分子生物学

背景情况:

  • 纳米孔测序提供实时,长读测序,但需要准确的基调,特别是对改性核酸.
  • 检测核酸修饰对于理解基因调节和细胞过程至关重要.

研究的目的:

  • 开发一种机器学习管道,用于使用纳米孔测序数据准确,单分子,单核酸检测核酸修饰.
  • 适应现有的基础调用算法,以增强修改检测.

主要方法:

  • 利用增量学习 (IL) 来改进富含修改序列的基础调用.
  • 在单个核酸上应用异常检测 (AD) 来识别修改状态.
  • 开发了一种用于序列无上下文修改检测的管道.

主要成果:

  • 通过使用控制寡头成功对管道进行了基准测试.
  • 将工作流应用于酵母tRNA,E.coli基因组DNA和人类mRNA.
  • 证明了N6-甲基氨酸 (m6A) 的跨物种检测和同时检测N1-甲基氨酸 (m1A) 和m6A.

结论:

  • IL-AD管道能够在单个分子分辨率下精确,无上下文检测核酸修饰.
  • 这种方法提升了纳米孔测序的能力,用于表观遗传学和表转录学研究.