揭示了FRG1在乳腺癌中的DNA修复作用
Shubhanjali Shubhanjali1,2, Talina Mohapatra1,2, Rehan Khan3
1School of Biological Sciences, National Institute of Science Education and Research, Room No. 204, PO: Jatani, Khurda, Bhubaneswar, Odisha, 752050, India.
Scientific reports
|August 21, 2024
概括
FRG1基因通过增强乳腺癌中的DNA修复作用,起到瘤抑制作用. 低FRG1水平会损害DNA修复,增加癌症风险并影响患者的存活率.
科学领域:
- 在瘤学瘤学.
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- FRG1 (FSHD区域基因1) 基因在乳腺癌和前列腺癌中起到瘤抑制作用.
- 基因组PARylation Factor 1 (HPF1) 对DNA修复至关重要,其表达与FRG1相关,这表明FRG1和DNA修复途径之间存在联系.
研究的目的:
- 研究FRG1在DNA修复机制中的作用,特别是基切除修复 (BER),以及其对乳腺癌的影响.
- 阐明FRG1影响的分子网络及其与瘤发生和患者结局的关联.
主要方法:
- 在分析包括差异基因表达,KEGG,GO和STRING数据库.
- 线性回归,qRT-PCR测试和性彗星测试以评估DNA损伤和修复.
- 考克斯回归用于风险得分计算和生存分析.
主要成果:
- FRG1与关键的BER基因具有正相关性,其缺乏与乳腺癌患者的TP53突变有关.
- 在乳腺癌细胞中,FRG1影响DNA基切除修复基因的转录.
- 降低FRG1水平会损害DNA修复能力,并与较差的整体存活率 (OS) 有关.
结论:
- 在乳腺癌细胞中,FRG1在维持DNA修复效率方面发挥着至关重要的作用.
- FRG1的调节失调会影响DNA修复途径,导致瘤发生并影响患者的预后.
- 向FRG1可能是乳腺癌的潜在治疗策略.
相关概念视频
Base Excision Repair
22.2K
One of the common DNA damages is the chemical alteration of single bases by alkylation, oxidation, or deamination. The altered bases cause mispairing and strand breakage during replication. This type of damage causes minimal change to the DNA double helix structure and can be repaired by the base excision repair (BER) pathways. BER corrects damaged DNA sequences by removing the damaged base and restoring the original base sequence using the complementary strand as a template.
The first step of...
The first step of...
22.2K
Nucleotide Excision Repair
3.5K
DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
3.5K
Long-patch Base Excision Repair
7.0K
Since the discovery of the two BER pathways, there has been a debate about how a cell chooses one pathway over the other and the factors determining this selection. Numerous in vitro experiments have pointed out multiple determinants for the sub-pathway selection. These are:
7.0K
Restarting Stalled Replication Forks
5.8K
DNA replication is initiated at sites containing predefined DNA sequences known as origins of replication. DNA is unwound at these sites by the minichromosome maintenance (MCM) helicase and other factors such as Cdc45 and the associated GINS complex.The unwound single strands are protected by replication protein A (RPA) until DNA polymerase starts synthesizing DNA at the 5’ end of the strand in the same direction as the replication fork. To prevent the replication fork from falling apart,...
5.8K
DNA Damage can Stall the Cell Cycle
9.1K
In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.1K
Homologous Recombination
50.3K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
50.3K


