相关实验视频

Updated: Jun 15, 2025

Intravitreal Injection and Quantitation of Infection Parameters in a Mouse Model of Bacterial Endophthalmitis

Intravitreal Injection and Quantitation of Infection Parameters in a Mouse Model of Bacterial Endophthalmitis

Published on: February 6, 2021

miR-27a-3p通过向TSC1促进感染性内炎的炎症反应

Yanting Chen¹, Shanxiang Li², Hong He²

¹Hainan Eye Hospital and Key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-Sen University, No.19 Xiuhua Road, Xiuying District, Haikou, 570311, Hainan, China. bery1455@126.com.

Scientific reports

|August 21, 2024

概括

微RNA-27a-3p通过向TSC1.1,使传染性内炎 (IE) 的炎症恶化. 这一发现为IE管理提供了新的治疗策略.

科学领域:

眼科医生眼科眼科
分子生物学分子生物学
免疫学免疫学免疫学

背景情况:

感染性内眼炎 (IE) 是一种严重的眼部疾病,威胁到视力.
微RNAs (miRNAs) 在调节炎症过程中起着至关重要的作用.
微RNA (miR) -27a-3p在IE病原发生中的特定作用仍然在很大程度上未被探索.

研究的目的:

研究miR-27a-3p对传染性内耳炎 (IE) 炎症的影响.
为了确定参与IE相关炎症的miR-27a-3p的目标基因.
探索针对MI治疗策略的miR-27a-3p的潜力.

主要方法:

建立了一种使用内脂多糖 (LPS) 注射的IE大鼠模型.
在老鼠和人类样本中使用RT-qPCR量化miR-27a-3p和炎症基因表达.
通过ELISA测量了人类玻璃体样本中的炎症性细胞因子度,并进行了体外研究以确定miR-27a-3p目标.

主要成果:

与对照组相比,在接受LPS治疗的老鼠和IE患者中,miR-27a-3p水平显著升高.
具有较高miR-27a-3p表达的患者表现出炎症性细胞因子水平的增加.
结核性硬化综合体1 (TSC1) 被确定为miR-27a-3p的直接向基因,其抑制促进了炎症.

结论:

miR-27a-3p通过向TSC1.1,加剧感染性内炎 (IE) 的炎症反应.
升高的miR-27a-3p是IE严重程度的潜在生物标志物.
向miR-27a-3p为管理IE提供了一个有希望的治疗途径.

关键词:

细胞因子 (cytokines) 是一种细胞因子.传染性内甲虫的感染.炎症反应是一种炎症反应.这就是TSC1的特点.在 miR-27a-3p 中.

更多相关视频

Induction of Ocular Surface Inflammation and Collection of Involved Tissues

Induction of Ocular Surface Inflammation and Collection of Involved Tissues

Published on: August 4, 2022

Primed Mycobacterial Uveitis PMU as a Model for Post-Infectious Uveitis

Primed Mycobacterial Uveitis PMU as a Model for Post-Infectious Uveitis

Published on: December 17, 2021

相关概念视频

The Tumor Microenvironment

The Tumor Microenvironment

Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...

T Cell Types and Functions

T Cell Types and Functions

When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...