综合生物信息学分析和实验验证揭示了ALOX5AP与质瘤的预后和免疫微环境之间的关系
Ping Song1, Hui Deng1, Yushu Liu1
1Department of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Hubei, Wuhan, 430030, P.R. China.
BMC medical genomics
|August 21, 2024
概括
阿拉基酸5-氧激酶激活蛋白 (ALOX5AP) 在质瘤中高度表达,特别是在M2巨中. 升高的ALOX5AP表明预后较差,并预测质瘤患者对免疫治疗的反应降低.
科学领域:
- 神经瘤学神经瘤学
- 癌症免疫学 癌症免疫学
- 分子生物学分子生物学
背景情况:
- 质瘤具有挑战性的初级中枢神经系统瘤.
- 阿拉基酸5-氧酶激活蛋白 (ALOX5AP) 与各种癌症的预后不佳有关.
- 对于ALOX5AP在质瘤预后和免疫微环境中的作用尚不清楚.
研究的目的:
- 调查ALOX5AP在质瘤中的预后价值.
- 探索ALOX5AP表达与质瘤免疫微环境之间的关系.
- 评估ALOX5AP作为质瘤免疫疗法反应的预测指标.
主要方法:
- 对公共数据库和质瘤组织微阵列的分析.
- 卡普兰-梅尔,考克斯回归和ROC分析用于预后价值.
- 使用ESTIMATE和CIBERSORT算法进行免疫透分析.
- 评估免疫疗法反应标志物和基因丰富分析.
- 多重复合免疫光染色用于ALOX5AP验证.
主要成果:
- ALOX5AP在质瘤中高度表达,与世卫组织等级,年龄,性别,IDH突变,1p19q共删除和MGMTp甲基化相关.
- ALOX5AP在巨细胞,单细胞和T细胞中表达,而不是瘤细胞.
- ALOX5AP表达与M2巨细胞透具有积极的相关性,并预测免疫治疗反应不佳.
- 高度质瘤和M2巨细胞中ALOX5AP的上调,通过免疫光检测证实.
结论:
- ALOX5AP在质瘤内的M2巨细胞中高度表达.
- ALOX5AP可以作为质瘤的预后生物标志物.
- ALOX5AP显示出作为预测质瘤免疫治疗反应的生物标志物的潜力.
相关概念视频
02:07Tumor Progression
Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
02:07Tumor Progression
Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...