适用于新生儿的小容量血小板缩剂更容易受到剪切诱导的储存损伤的影响
Dean Pym1,2, Amanda J Davies1, Jessica O Williams1
1Centre of Cardiovascular Health and Ageing, Cardiff Metropolitan University, Cardiff, Wales, UK.
Platelets
|August 22, 2024
概括
新生儿血小板储存袋经历了更高的剪切应力,减少了功能. 将激发率降低到每分钟40转,可以改善血小板功能,并减少新生儿输血储存期间的激活.
科学领域:
- 生物物理学的生物物理.
- 血液学 血液学 血液学
- 输血医学 输血医学
背景情况:
- 血小板储存损伤 (PSL) 影响储存的血小板功能.
- 降低温度储存得到了很好的研究,但储存引起的切割应力被忽视.
- 新生儿血小板度 (PCs) 特别容易受到存储损伤.
研究的目的:
- 调查是否最小化激动诱导的剪切应力可以改善存储的新生儿PC的功能.
- 为了比较新生儿与成人PC存储袋中的剪切应力和血小板功能.
- 为了确定新生儿PC存储的最佳动频率.
主要方法:
- 颗粒跟踪分析用于测量新生儿和成人PC袋中的液体运动,在不同的振动频率 (20-60rpm) 上.
- 对存储8天的血小板进行生物化学分析,聚合试验和激活标记表达分析.
- 在不同激发条件下的血小板功能和激活的比较.
主要成果:
- 新生儿PC体验的储存诱导的剪压明显高于成年PC.
- 较高的剪切应力导致新生儿PC功能减少,并且从第二天开始激活.
- 与60rpm相比,在储存期间以40rpm的动改善了新生儿PC功能,激活率降低.
结论:
- 为新生儿使用的小容量PC存储通过剪切应力诱导促进PSL.
- 目前推的激发频率可能对新生儿PCs来说不是最佳的.
- 建议进一步评估激发协议或按需生产新生儿PC.
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