鉴定关键基因与氧化应激途径在诱导的血液毒性的关键基因
Jin He1, Cheng Peng2, XiaoHan Yang1
1Shandong Academy of Occupational Health and Occupational Medicine & Shandong Provincial Occupational Diseases Hospital, Shandong First Medical University & Shandong Academy of Medical Sciences, Shandong, China.
Heliyon
|August 22, 2024
概括
研究人员确定了素诱导的骨髓衰竭 (BMF) 的新生物标志物. Mapk11和Fgf3显示为人类血毒性的诊断指标.
科学领域:
- 血液学 血液学 血液学
- 毒理学 毒理学 毒理学
- 基因组学就是基因组学.
背景情况:
- 暴露会导致慢性骨髓衰竭 (BMF),是一种血液毒性.
- 目前对BMF的诊断方法有限,需要确定可靠的生物标志物.
- 异常基因表达和氧化应激与BMF发展有关.
研究的目的:
- 通过差异基因表达 (DEG) 分析识别素诱导的BMF的潜在生物标志物.
- 为了验证候选基因作为甲血毒性的诊断标记.
主要方法:
- 开发了一种由素诱导的BMF的小鼠模型.
- 利用RNA测序 (RNA-seq) 来识别骨髓细胞中的DEG.
- 进行了基因和基因组京都百科全书 (KEGG) 途径分析.
- 在小鼠和人类样本中使用定量实时PCR (qRT-PCR) 验证的候选基因.
主要成果:
- 确定了十种统计学上显著的DEG,主要与线素激活蛋白激酶 (MAPK) 氧化应激途径相关.
- 在BMF小鼠中,包括Mapk11,Foxo1,Bank1,Lefty1,Ren1,P2rx7和Fgf3 (表达增加) 以及Cdc42ep2 (表达减少) 在内的8个DEG被显著改变.
- 在中毒患者的外周血液单核细胞中验证了八个候选基因.
结论:
- Mapk11和Fgf3成为人类甲血毒性新生物标志物的主要候选基因.
- 这项研究为早期诊断和管理诱导的BMF提供了新的候选基因.
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