蜜蜂产品对羊卵细胞在体外成熟的改善作用
Aaishah Kaabi1, Ibrahim Barakat2, Reem Alajmi1
1Zoology Department, College of Science, King Saud University, Riyadh, Kingdom of Saudi Arabia.
Veterinarni medicina
|August 22, 2024
概括
低度的尼日利亚三蜂蜜,沙特西德蜂蜜和蜜蜂花粉在体外增强了绵羊卵细胞的成熟. 这些天然产品改善了成熟率,谷氨水平和基因表达,为改善辅助生殖技术提供了潜力.
科学领域:
- 生殖生物学 生殖生物学
- 卵细胞成熟的过程
- 在体外受精过程中受精.
背景情况:
- 卵细胞成熟对于成功受精和胚胎发育至关重要.
- 改善体外成熟 (IVM) 协议对于改善辅助生殖技术至关重要.
- 自然化合物越来越多地被探索,因为它们有改善生殖结果的潜力.
研究的目的:
- 调查尼格拉三蜂蜜,沙特西德蜂蜜和蜜蜂花粉对羊卵细胞体外成熟 (IVM) 的影响.
- 评估这些天然产品对卵细胞成熟率,谷氨酸度和关键基因表达的影响.
主要方法:
- 在IVM介质中培养不成熟的绵羊卵细胞,并补充了不同度的尼日利亚三蜂蜜,沙特西德蜂蜜或蜜蜂花粉,持续24小时.
- 评估了成熟率 (二次元期阶段),谷氨 (GSH) 水平和基因表达 (GDF-9,MPF,CMOS,IGF-1,BAX).
主要成果:
- 与对照组相比,较低度 (5%蜂蜜,1μg/ml花粉) 显著增加了卵细胞成熟率.
- 尼日利亚三蜂蜜显著提高了谷氨酸水平.
- 沙特西德蜂蜜表明,对于大多数研究的基因,它具有最有利的基因表达特征.
结论:
- 低剂量补充Nigella sativa蜂蜜,沙特西德蜂蜜和蜜蜂花粉对羊卵细胞IVM产生积极影响.
- 这些天然产品增强成熟率,抗氧化能力 (GSH) 和特定基因表达,表明它们在改善羊卵细胞质量方面具有潜在的实用性.
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