主要多步的基中继激活包括细胞因素和非生物应激反应:从比较分析Brassica类型A反应调节者的见解
Katrina Leslie Nicolas Mala1,2, Jan Skalak1,2, Elena Zemlyanskaya3,4
1CEITEC - Central European Institute of Technology, Masaryk University, Kamenice 5/A2, 625 00 Brno, Czech Republic.
Journal of experimental botany
|August 22, 2024
概括
这项研究确定了Brassica物种中的新型A型响应调节器 (RRA) 基因,揭示了它们在植物发育和对细胞因素和压力的适应性反应中的保留作用. 我们在寒冷压力诱导的RRA基因表达中发现了部分细胞因素依赖.
科学领域:
- 植物分子生物学 植物分子生物学
- 植物信号通道的信号通道.
- 进化基因组学是进化的基因组学.
背景情况:
- 多级层 (MSP) 信号集成了植物发育和适应的荷尔蒙和环境线索.
- 类型A响应调节器 (RRA) 基因是MSP信号的关键下游组件,主要参与负反调节.
- 现有的数据表明,RRA基因也在植物应激反应中发挥作用.
研究的目的:
- 在Brassica oleracea和Brassica napus中使用进化保护来识别新的假定RRA基因.
- 调查布拉西卡科 (Brassicaceae) 中的RRA基因的遗传关系和进化压力.
- 描述RRA基因的细胞因素介导上调调节动力学,并探索它们在应激反应中的保留作用.
主要方法:
- 进行比较基因组学和遗传学分析,以确定Brassica物种中的RRA同类物种.
- 对基因表达模式的分析,以应对细胞激素和非生物压力.
- 使用Arabidopsis thaliana TCSv2报告员系统来评估保存的监管机制.
主要成果:
- 在Brassica oleracea中确定了5个新的RRA基因,在Brassica napus中确定了38个.
- 遗传学分析揭示了基因特异性的选择性压力,ARR3,ARR6和ARR16同类基因被维持为单个基因.
- 在Brassicaceae中观察到细胞因素介导的RRA基因上调的保存和特定物种模式.
- 在B. rapa和B. napus中证明了细胞因素和压力介导的RRA基因上调的保存机制.
- 发现了对寒冷压力诱导的RRA转录的部分细胞因素依赖性.
结论:
- 这项研究扩大了Brassica物种中已知的RRA基因库,为它们的进化历史提供了洞察力.
- RRA基因在调解植物对细胞因素和非生物压力的反应方面表现出保守的功能,突出显示了这些途径的相互联系.
- 细胞因素信号传递在作物对寒冷压力等环境挑战的适应性反应中起着至关重要的作用.
相关概念视频
Cell Signaling in Plants
5.6K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.6K
MAPK Signaling Cascades
5.3K
Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...
5.3K
TGF - β Signaling Pathway
7.3K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
7.3K
Interactions Between Signaling Pathways
6.2K
Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.2K
Photoreceptors and Plant Responses to Light
20.2K
Light plays a significant role in regulating the growth and development of plants. In addition to providing energy for photosynthesis, light provides other important cues to regulate a range of developmental and physiological responses in plants.
20.2K
Prokaryotic Transcriptional Activators and Repressors
20.9K
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
Transcription of prokaryotic...
20.9K


