通过与瘤衍生的细胞外囊泡杂交来最大限度地提高脂质体瘤的输送
Shoukath Sulthana1, Dinesh Shrestha1, Santosh Aryal1
1Department of Pharmaceutical Sciences and Health Outcomes, The Ben and Maytee Fisch College of Pharmacy, University of Texas at Tyler, Tyler, TX 75799, USA. santosharyal@uttyler.edu.
Nanoscale
|August 22, 2024
概括
研究人员将合成脂质体与来自乳腺癌细胞的细胞外囊泡 (EVs) 进行杂交,创造了向输送器 (LEVs). 这些LEV显示在临床前模型中增强了瘤向和转移检测.
科学领域:
- 生物技术和纳米医学
- 癌症研究 癌症研究
- 药物输送系统 药物输送系统
背景情况:
- 细胞外囊泡 (EVs) 在细胞间通信中至关重要,并具有诊断和治疗癌症等疾病的潜力.
- 目前生物系统内EV导航的局限性阻碍了它们的治疗应用.
- 用合成脂质体混合化EVs可以克服这些挑战,以实现有针对性的交付.
研究的目的:
- 从4T1小鼠乳腺癌细胞中混合合成脂质体与EV,以创建有针对性的输送结构 (LEV).
- 描述开发的LEVs的物理化学特性和稳定性.
- 在临床前乳腺癌模型中评估LEVs的in vivo瘤向和转移检测能力.
主要方法:
- 使用膜挤出,混合化EVs与合成脂质体.
- 描述LEV的大小,单分散性和稳定性.
- 通过免疫栓塞和ELISA对EV特异性标记物 (CD63,CD9,CD81) 的量化.
- 在携带4T1正型乳腺瘤的小鼠中LEV分布的体内成像.
主要成果:
- 成功形成单分散的LEV,平均水力动力学尺寸为140 ± 5nm.
- 在LEV中确认了EV特异性蛋白质的存在,确保了可复制性.
- 与对照组相比,在瘤和器官 (肝脏,脏,肺部) 中证明了最大LEV吸收.
- 使用ex vivo成像,可视化了肺转移中的LEV积累.
结论:
- 混合LEV在临床前乳腺癌模型中表现出优越的瘤向和转移检测能力.
- 这项研究提出了一个有前途的战略,用于开发具有增强目标识别的先进药物输送解决方案.
- LEV技术为改善癌症诊断和治疗提供了潜在的途径.
相关概念视频
02:17The Tumor Microenvironment
6.3K
Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
6.3K
02:30Metastasis
5.5K
Metastasis is the spread of cancer cells from the original site to distant locations in the body. Cancer cells can spread via blood vessels (hematogenous) as well as lymph vessels in the body.
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
5.5K
01:27Tumor Immunotherapy
2.6K
Immunotherapy is a treatment that boosts or manipulates the immune system to fight diseases, including cancer. For instance, by stimulating an immune response through vaccinations against viruses that cause cancers, like hepatitis B virus and human papillomavirus, these diseases can be prevented. Nonetheless, some cancer cells can avoid the immune system due to their rapid mutation and division. The immune response to many cancers involves three phases: elimination, equilibrium, and escape.
2.6K