多重DNA病毒和HPV集成在逆转乳头瘤和相关的鼻癌
Maria K Jauhiainen1,2,3,4, Lari Pyöriä2, Sanna Viitasalo1
1Department of Otorhinolaryngology - Head and Neck Surgery, Helsinki University Hospital and University of Helsinki, Helsinki, Finland.
The Laryngoscope
|August 22, 2024
概括
鼻腔逆转乳头瘤 (IPs) 携带多种DNA病毒,包括人类乳头瘤病毒 (HPV). HPV16的存在与IPs的恶性转变密切相关,这表明它在IP相关的鼻腔状细胞癌 (IP-SNSCC) 中发挥了作用.
科学领域:
- 耳鼻喉科 耳鼻喉科 耳鼻喉科
- 病毒学 病毒学
- 在瘤学瘤学.
背景情况:
- 鼻逆向乳头瘤 (IP) 呈现出局部侵略性生长,复发潜力和恶性转变风险.
- 人类乳头瘤病毒 (HPV) -6和 -16经常在IP中发现,但其他DNA病毒的作用仍未得到充分探索.
- 调查病毒存在和HPV整合对于理解IP进展到IP相关的鼻腔状细胞癌 (IP-SNSCC) 至关重要.
研究的目的:
- 在鼻逆 papiloma (IP) 样本中识别和描述各种DNA病毒.
- 研究人类乳头瘤病毒 (HPV) 基因组的整合到宿主基因组.
- 在前恶性和恶性IP转变阶段分析HPV变异.
主要方法:
- 分析了28个人的35个IP样本,其中6个是IP-SNSCC.
- 定量PCR (qPCR) 用于查16种DNA病毒 (疹病毒,帕尔沃病毒,多重病毒).
- 针对性下一代测序 (NGS) 进行深入的HPV分析,包括整合和变种识别.
主要成果:
- 在46%的患者中检测到疹病毒,帕沃病毒和多种病毒,21%的患者有多种感染.
- 在IP-SNSCC和先前的良性IP样本中发现了HPV16DNA,这表明HPV16在恶性转变中发挥了作用.
- 确定了HPV6和HPV16融入宿主基因组的证据,以及轻微的类型变异.
结论:
- 鼻腔逆转乳头瘤经常含有多个DNA病毒.
- 检测HPV16仅与IP-SNSCC或随后转变为恶性的IP有关.
- 这些发现凸显了HPV16在鼻癌发生过程中的潜在瘤作用.
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