MAF bZIP转录因子B的表达通过抑制NF-κB通路来保护性结肠炎
Jingwen Li1, Qingmin Li2,3, Wei Ma2
1Department of Gastroenterology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China.
Immunity, inflammation and disease
|August 22, 2024
概括
上调MAF bZIP转录因子B (MAFB) 通过通过NF-κB通路减少炎症和恢复肠道屏障功能来保护性结肠炎 (UC).
科学领域:
- 胃肠病学 胃肠病学
- 分子生物学分子生物学
- 免疫学 免疫学 免疫学
背景情况:
- 性结肠炎 (UC) 是一种具有复杂病原性的慢性炎症性肠病.
- 目前对UC的治疗有局限性,需要探索新的治疗点.
- 对于MAF bZIP转录因子B (MAFB) 在UC病变发生中的作用尚不清楚.
研究的目的:
- 在性结肠炎 (UC) 的小鼠模型中研究MAF bZIP转录因子B (MAFB) 的治疗潜力.
- 探索MAFB对UC炎症,氧化应激和肠道屏障完整性的影响.
- 阐明MAFB的作用背后的分子机制,特别是其与NF-κB通路的相互作用.
主要方法:
- 建立了UC的体内 (诱导的硫酸德克斯) 和体内 (LPS诱导的IEC-6细胞) 模型.
- 评估疾病严重程度,结肠长度,肠道通透性 (FITC-Dextran) 和组织病理学 (H&E染色).
- 量化炎症性细胞因子 (IL-1β,IL-6,TNF-α,IL-10),氧化应激标记物 (MDA,SOD,CAT,GSH),紧结蛋白和NF-κB通路蛋白 (西斑,qRT-PCR,免疫光).
主要成果:
- 在DSS诱导的大肠炎小鼠中,MAFB水平下调.
- 通过抑制炎症,氧化应激和肠道屏障损伤,MAFB上调改善了DSS诱导的大肠炎.
- 在IEC-6细胞中,MAFB过度表达抑制了LPS诱导的炎症和上皮屏障功能障碍.
- 在体外和体外模型中,MAFB上调抑制了NF-κB通路.
结论:
- MAFB的上调显示出对性结肠炎的保护作用.
- MAFB通过抑制炎症和肠道屏障损伤来减轻UC.
- 抑制NF-κB通路是MAFB在UC中发挥治疗作用的关键机制.
相关概念视频
NF-κB-dependent Signaling Pathway
7.4K
The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
NF-κB-dependent Signaling Mechanism
The...
7.4K
Inflammatory Bowel Disease I: Ulcerative Colitis
159
Introduction
Inflammatory bowel disease, or IBD, encompasses a group of disorders characterized by chronic inflammation or ulceration of the gastrointestinal tract.
Risk Factors
The exact cause of IBD remains unclear, although it is believed to be due to a mix of genetic, environmental, microbial, and immune factors. Genetic factors are significant in determining susceptibility to IBD, with family history being a critical risk factor. Individuals with a first-degree relative who has IBD are at...
Inflammatory bowel disease, or IBD, encompasses a group of disorders characterized by chronic inflammation or ulceration of the gastrointestinal tract.
Risk Factors
The exact cause of IBD remains unclear, although it is believed to be due to a mix of genetic, environmental, microbial, and immune factors. Genetic factors are significant in determining susceptibility to IBD, with family history being a critical risk factor. Individuals with a first-degree relative who has IBD are at...
159
Drugs for Treatment of Ulcerative Colitis in IBD
130
Ulcerative colitis is a chronic inflammatory condition primarily affecting the colon and rectum. The primary drugs used in the treatment of ulcerative colitis are aminosalicylates. They exhibit anti-inflammatory and immunosuppressive properties. They modulate inflammatory mediators and inhibit the activity of nuclear factor κB (NF-κB). Aminosalicylates also reduce inflammation by inhibiting prostaglandin and leukotriene production and decreasing neutrophil chemotaxis and superoxide...
130
Master Transcription Regulators
6.9K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
6.9K
Co-activators and Co-repressors
7.3K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
7.3K
Abnormal Proliferation
4.5K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.5K


