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Published on: June 23, 2014
周围血液单核细胞分泌自身抗体的不同模式在自身免疫性节节病症中
Sophia Rohrbacher1, Sabine Seefried1, Beate Hartmannsberger1
1From the Department of Neurology (S.R., S.S., B.H., L.A., C. Sommer, K.D.); Department of Anaesthesiology, Intensive Care, Emergency and Pain Medicine Centre for Interdisciplinary Pain Medicine (B.H.); Institute of Experimental Biomedicine (R.A., A.Z.), University Hospital Würzburg; German Center for Neurodegenerative Diseases (DZNE) Berlin (F.A.A., H.P.); Department of Neurology and Experimental Neurology, Charité-Universitätsmedizin Berlin, Corporate Member of Freie Universität Berlin, Humboldt-Universität Berlin; Section Translational Neuroimmunology (D.B., C.G.), Department of Neurology, Jena University Hospital; Department of Neurology (C.D.), University Medical Center of the Johannes Gutenberg University, Mainz; Department of Neurology (J.D., Z.E.), University Hospital Ulm; Department of Neurology (T.H., H.T.), Therapiezentrum Burgau; Department of Neurology (S.K.), Friedrich Baur Institute, LMU University Hospital, LMU Munich; Department of Neurology (M. Marziniak), Kbo-Isar-Amper-Hospital Munich East; Department of Neurology (M. Mäurer), Klinikum Würzburg Mitte gGmbH, Standort Juliusspital; Department of Neurology (F.S.), LMU University Hospital, LMU, Munich; Department of Neurology (B.S.), DKD HELIOS Klinik Wiesbaden; Department of Paediatric and Adolescent Medicine (C. Steen), St Joseph Hospital, Berlin; Department of Neurology (A.T.), HELIOS Klinikum Erfurt; and Neurologische Praxis Dres. Wessely (L.W.), Menden, Germany.
预测自身免疫性结节病的过程和rituximab的反应是关键. 周围血液细胞在体外自身抗体的产生可以表明患有特定自身抗体的患者的疾病轨迹和治疗成功.
科学领域:
- 神经免疫学 神经免疫学
- 自免疫性疾病 自免疫性疾病
- 神经学 神经学
背景情况:
- 针对偏节蛋白的自身免疫结节病导致严重的感觉运动神经病变.
- 对利图西马布的治疗反应有所不同,因此需要预测性标志物.
- 了解疾病的过程和治疗反应对于患者的管理至关重要.
研究的目的:
- 调查外周血液单核细胞的体外自身抗体分泌是否预测自身免疫结节病的疾病过程和rituximab反应.
- 识别与不同临床表型和治疗结果相关的不同的B细胞反应.
主要方法:
- 在试验室中刺激了外围血液单核细胞,以测量自身抗体分泌.
- 血清自身抗体标位与体外生产一起分析.
- 关于疾病进展和对rituximab的反应的临床数据与B细胞反应相关.
主要成果:
- 在大多数患有抗Neurofascin-155,抗Contactin-1和抗Caspr1 IgG4自身抗体的患者中检测到体外自身抗体的产生,与良好的rituximab反应相关.
- 一个具有抗Neurofascin-155 IgG4和较差rituximab反应的亚组没有 in vitro 产生,这表明外周B细胞参与.
- 患有抗泛神经素自身抗体和单相性疾病的患者缺乏体外生产,表明缺乏特异性记忆B细胞.
结论:
- 在自身免疫结节病症中,不同的B细胞反应可以作为疾病进程的预测标志物.
- 在体外自身抗体分泌模式可以预测rituximab的疗效.
- 这些发现有助于优化自身免疫结节病的治疗策略.
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