在饮用水分配系统的维修后,替代HPC22的替代方案
Marcelle J van der Waals1, Nikki van Bel1, Frits van Charante1
1KWR Water Research Institute, Groningenhaven 7, Nieuwegein, 3433 PE, the Netherlands.
Water research
|August 22, 2024
概括
像腺三酸盐 (ATP) 和酶活性等快速方法在检测饮用水中的微生物污染方面表现有前途,为传统培养提供了更快的替代方案. 这些可靠的方法可以在饮用水分配系统维护期间加强公共卫生保护.
科学领域:
- 环境微生物学环境微生物学
- 分析水质,分析水质.
- 公共卫生 公共卫生
背景情况:
- 饮用水配送系统 (DWDS) 在维护或管道漏洞期间可能受到外部来源的污染.
- 微生物污染对公众健康构成重大风险.
- 传统的微生物学测试 (例如,HPC22) 是耗时的.
研究的目的:
- 评估快速微生物检测方法作为饮用水质量评估中传统培养的替代方法.
- 为了比较腺三酸盐 (ATP) 的可靠性和灵敏性,流动细胞计量,酶活性和qPCR与22°C (HPC22) 的异位变异板计数进行比较.
主要方法:
- 四种快速检测方法的比较:腺三酸盐 (ATP总实验室,ATP细胞群),流细胞计,酶活性 (ALP-2) 和定量聚合酶连锁反应 (qPCR).
- 使用稀释系列和饮用水分配系统的现场样本进行验证.
- 对快速方法与传统HPC22计数之间的相关性进行统计分析.
主要成果:
- 与流细胞计和qPCR相比,ATP (ATP总实验室,ATP细胞流动) 和酶活性 (ALP-2) 在检测微生物污染方面表现出更高的可靠性和灵敏性.
- 在HPC22和ATP/酶活性/qPCR方法之间观察到显著的相关性 (p < 0.05,R2 = 0.61-0.76),而流细胞计显示较弱的相关性 (R2 = 0.24-0.52).
- 来自DWDS维护后的水样通常显示HPC22的数量很低,通常低于1000 CFU/mL.
结论:
- 在DWDS维护后,ATPtotal-lab,ATPcell-mob和ALP-2是监测饮用水微生物质量的有希望的快速替代方案.
- 建议进行进一步的实地研究,以验证和比较这些快速方法,以潜在地取代HPC22分析.
- 实施更快,更可靠的方法可以提高确保安全饮用水供应的效率.
更多相关视频
12:11A Modified EPA Method 1623 that Uses Tangential Flow Hollow-fiber Ultrafiltration and Heat Dissociation Steps to Detect Waterborne Cryptosporidium and Giardia spp.
Published on: July 9, 2012
20.4K
07:00Failure of Cleaning Verification in Pharmaceutical Industry Due to Uncleanliness of Stainless Steel Surface
Published on: August 11, 2017
8.2K
相关概念视频
Multiple Pipe Systems
728
Multipipe systems consist of complex configurations of interconnected pipes designed to transport fluids efficiently across intricate networks. They are essential in engineering applications requiring precise control over flow distribution, pressure, and head loss. They are categorized into series, parallel, loop, and network configurations, each distinguished by unique flow characteristics and applications.
Series Configuration
In a series configuration, fluid flows sequentially from one pipe...
Series Configuration
In a series configuration, fluid flows sequentially from one pipe...
728
Precipitation and Co-precipitation
1.7K
Precipitation and coprecipitation methods can be used to separate a mixture of ions in a solution. In qualitative inorganic analysis, ions that form sparingly soluble precipitates with the same reagent are separated based on the differences in solubility products. For example, consider the separation of Cu(II) and Fe(II) ions by precipitation as insoluble sulfides. First, copper(II) sulfide is precipitated by the addition of acidic H2S, where the dissociation of H2S is suppressed. Adding H2S...
1.7K
Coagulation
278
Colloidal solids are solid particles suspended in solution. They are usually negatively charged, attracting a compact primary layer of positively charged ions, which attract more counterions to form an electrical double layer. Electrostatic repulsion between the charged double layers prevents the particles from colliding, stabilizing the colloids. These solids are often undesirable because they can contain toxins that are difficult to remove. Coagulation is a technique that helps aggregate and...
278
High-Performance Liquid Chromatography: Elution Process
442
In High-Performance Liquid Chromatography (HPLC), the elution process is critical to the separation of analytes and the quality of chromatographic results. Elution describes how compounds move through the column and separate based on their interactions with the mobile and stationary phases. This process determines the resolution, peak shape, and retention times in the chromatogram, which are essential for identifying and quantifying components in complex mixtures. Understanding the elution...
442
Single Pipe Systems
120
In pipe flow analysis, problems are typically categorized into three types — Type I, Type II, and Type III — based on the known parameters and the desired outcome. Each type of problem addresses specific engineering requirements using fluid properties, pipe characteristics, and operational conditions.
In a Type I problem, fluid properties (density and viscosity), pipe characteristics (including diameter, length, and surface roughness), and the flow rate or average velocity are...
In a Type I problem, fluid properties (density and viscosity), pipe characteristics (including diameter, length, and surface roughness), and the flow rate or average velocity are...
120
