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在规范性衰老研究中,细胞外微RNA与精神症状相关

Xinye Qiu¹, Mahdieh Danesh Yazdi², Cuicui Wang¹

¹Department of Environmental Health, Harvard T.H. Chan School of Public Health, Boston, MA, USA.

Journal of psychiatric research

|August 22, 2024

概括

细胞外微RNAs (ex-miRNAs) 显示出作为老年人精神症状的生物标志物的潜力. 这项研究确定了与各种心理健康状况相关的特定的ex-miRNAs,表明它们在亚临床疾病检测中的作用.

科学领域:

生物化学生物化学
遗传学遗传学是一个
精神病学是一个精神病学.

背景情况:

微RNAs (miRNAs) 是神经系统疾病的已知的生物标志物,但它们在精神疾病中的作用仍未得到充分探索.
关于细胞外miRNAs (ex-miRNAs) 与精神病症状之间的关联的证据有限,特别是在老年人群中.

研究的目的:

为了研究前-miRNAs和老年成年男性的精神症状之间的关联.
识别特定的前-miRNAs,目标基因和与精神健康相关的生物途径.
探索前miRNAs作为亚临床精神疾病的生物标志物的潜力.

主要方法:

使用了569名老年成年男性的规范衰老研究 (NAS) 队列.
使用简要症状目录 (BSI) 测量精神症状,并进行重复评估.
从使用小RNA测序的血细胞外囊泡中对前miRNA进行了分析.
采用线性混合效应模型,包括LASSO,以评估395个前miRNA和精神症状之间的关联.
进行了途径分析,以探索已识别的miRNAs的生物功能.

主要成果:

确定了与全球和维度精神病症状尺度相关的特定的前miRNAs.
hsa-miR-320d连续与两个全球汇率指标尺度联系在一起.
在不同的症状得分中发现了重叠的miRNA,包括hsa-miR-379-3p和hsa-miR-151a-5p.
最重要的相关途径包括p53信号传递,河马信号传递以及与癌症和神经疾病相关的途径.

结论:

提供了早期证据,支持前-miRNAs与老年人精神疾病之间的联系.
表明前miRNAs可能作为检测亚临床精神疾病的有价值的生物标志物.
突出了ex-miRNA分析的潜力,以了解精神病症状的生物学基础.

关键词:

生物途径生物途径生物标志物生物标志物细胞外的微RNAs.精神病医疗健康精神病医疗健康

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MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA ends...

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