中等pH对酵母血潜力的影响
Antonio Peña1, Norma Silvia Sánchez1, Yazmín Ramiro-Cortés2
1Departamento de Genética Molecular, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Circuito Exterior s/n, Ciudad Universitaria, 04510, México City, Mexico.
Archives of biochemistry and biophysics
|August 22, 2024
概括
酵母血潜力 (PMP) 在低pH下保持,使用黄素T (ThT) 光. 这种染料有效地测量了PMP,这对于酵母在发酵等酸性环境中生存至关重要.
科学领域:
- 生物化学和分子生物学
- 微生物学 微生物学
背景情况:
- 酵母细胞必须保持血潜力 (PMP) 才能生存,特别是在酸性环境中.
- 发酵过程经常使酵母暴露在低pH条件下.
研究的目的:
- 为了研究pH和离子 (K+) 对酵母PMP的影响.
- 为了评估 thioflavin T (ThT) 作为测量 PMP 的可靠染料.
主要方法:
- 利用ThT的光变化,在pH值为4.0,6.0和7.0时对PMP进行定性评估.
- 量化测量了ThT积累,并应用了Nernst方程来估计mV中的PMP值.
- 研究了二碳酸盐在发酵期间在PMP建立中的作用.
主要成果:
- 在pH4.0的细胞中,PMP值低于,但接近于在pH6.0和7.0观察到的PMP值.
- 使用ThT积累和与光观测一致的Nernst方程估计的PMP值.
- 证明了THT在质量和数量PMP评估中的有效性.
结论:
- 酵母在一系列pH值中保持功能性PMP,这对于在酸性条件下生存至关重要.
- 提奥夫拉T是一种多功能且有效的染料,用于研究酵母血潜力.
- 乙碳酸盐有助于在发酵过程中在酵母中建立PMP.
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