相关实验视频

Updated: May 11, 2026

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence
11:51

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence

Published on: April 27, 2018

11.9K

通过合理设计,在Corynebacterium glutamicum的分泌过程中增强ApxII抗原的分子稳定性

Xiuxia Liu1, Shujie Yang1, Manman Sun2

  • 1Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China; National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi 214112, China; Jiangsu Provincial Research Center for Bioactive Product Processing Technology, Jiangnan University, Wuxi 214122, China.

Journal of biotechnology
|August 22, 2024
PubMed
概括

研究人员提高了ApxII的稳定性,这是猪传染性肺炎的疫苗抗原. R114E/T115D变种表现出更好的稳定性和活性,为养猪业的工业应用提供了潜力.

关键词:
在Corynebacterium glutamicum的研究中,我们发现分子稳定性朝着蛋白质分解性降解的方向.理性的设计理性的设计.截断的 ApxII 截断的

更多相关视频

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids11:56

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids

Published on: May 4, 2018

12.4K
Homogeneous Glycoconjugate Produced by Combined Unnatural Amino Acid Incorporation and Click-Chemistry for Vaccine Purposes13:53

Homogeneous Glycoconjugate Produced by Combined Unnatural Amino Acid Incorporation and Click-Chemistry for Vaccine Purposes

Published on: December 19, 2020

5.0K

相关概念视频

Production of Pharmaceuticals01:30

Production of Pharmaceuticals

Industrial insulin production uses genetically engineered E. coli expressing a proinsulin gene controlled by a tryptophan promoter and containing a methionine linker for later cleavage. The cells also carry ampicillin resistance for selective growth. Seed cultures are stored at −80 °C and production begins by thawing a small amount to inoculate starter cultures, which are progressively scaled to a 50,000-L bioreactor. In the bioreactor, E. coli grow in nutrient-rich media under sterile, tightly...