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黑鱼的SRP54负面调节MDA5介导的抗病毒天生的免疫力

Jixiang Chu¹, Yixia Chen¹, Yanfang Wu¹

¹State Key Laboratory of Developmental Biology of Freshwater Fish, College of Life Science, Hunan Normal University, Changsha, 410081, China.

Developmental and comparative immunology

|August 22, 2024

概括

信号识别颗粒54 kDa (SRP54) 通过抑制MDA5-MAVS相互作用来负面调节teleost抗病毒免疫力. 击败SRP54增强了宿主抗病毒防御,揭示了它在天生的免疫力中的作用.

科学领域:

分子生物学分子生物学
免疫学免疫学免疫学
鱼类生物学鱼类生物学

背景情况:

信号识别粒子54 kDa (SRP54) 是参与蛋白质运输的SRP复合物的关键组成部分.
在哺乳动物中,SRP54负面调节RIG-I类受体 (RLR) 信号通路.
目前尚不清楚SRP54在远程抗病毒天生的免疫力中的作用.

研究的目的:

为了克隆和描述黑鱼SRP54同类物 (bcSRP54).
为了阐明bcSRP54在teleost抗病毒天生的免疫力中的作用.
研究bcSRP54调节MDA5信号通路的分子机制.

主要方法:

基因克隆和bcSRP54.4的基因测序
免疫光 (IF) 用于亚细胞定位.
报告员测试以评估干扰素 (IFN) 促进剂活性.
同表达和敲击实验来评估抗病毒活性.
同免疫沉 (co-IP) 用于确定蛋白质相互作用.

主要成果:

bcSRP54被克隆,由1515个编码504个氨基酸的核酸组成,并定位到细胞质中.
过度表达bcSRP54抑制了MDA5介导的IFN信号传递和抗病毒活性.
bcSRP54与bcMDA5相互作用,并阻碍了bcMDA5与bcMAVS的结合.
击败bcSRP54增强了宿主抗病毒防御.

结论:

bcSRP54 负面调节 teleost 抗病毒天生的免疫力.
bcSRP54通过准bcMDA5和破坏bcMDA5-bcMAVS相互作用来抑制IFN信号传递.
这项研究提供了关于SRP54在远距离IFN信号传输和抗病毒防御中的作用的见解.

关键词:

黑鱼是一种黑鱼.这是天生的免疫力.MDA5 MDA5 MDA5 是一个简单的数字.在SRP54中使用SRP54.在SVCV中,SVCV是最重要的.

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